Wayne:High Throughput Sequencing Resources: Difference between revisions
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== Basic server commands (for Sirius) == | == Basic server commands (for Sirius) == | ||
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<td><b>Command</b></td> | <td><b>Command</b></td> |
Revision as of 17:13, 15 February 2013
High throughput (HT) platform and read types
- Illumina single-end vs. paired-end
- 454 Roche
- SOLiD
- MiSeq
- Ion Torrent
File formats and conversions
- bcl
- qseq
- fastq
Deplexing using barcoded sequence tags
- Editing (or hamming) distance
Quality control
- Fastx tools
- Using mapping as the quality control for reads
Trimming and clipping
- Trim based on low quality scored per nucleotide position within a read
- Clip sequence artefacts (e.g. adapters, primers)
DNA sequence analysis
RNA-seq analysis
- Quantifying and annotating aligned reads
- DESeq
- edgeR
A variety of additional R packages are available for normalizing RNA-Seq read count data and identifying differentially expressed genes (DEG):
- easyRNASeq (simplifies read counting per genome feature)
- DEXSeq (Inference of differential exon usage)
- DEGseq
- baySeq (also see: segmentSeq)
- Genominator (Bullard et al. 2010)
Basic server commands (for Sirius)
Command | Usage |
pwd | Print working directory (your current location |
ls | List (all contents of current location) |
ls flags | ls -a (hidden files), ls -l (long/detailed list), ls -t (sorted by time modified instead of name) |
cd | Change directories |
mkdir | Make directories |
head filename | Print to screen the top 10 lines or so of the specified file |
tail filename | Print to screen the last 10 lines or so of the specified file |
more filename | Allows file contents or piped output to be sent to the screen one page at a time |
less filename | Opposite of more command |
whereis [filename, command, etc] | Lists all occurances of filename or command |
mv | Move (akin to cut/paste), to remove the file in the current location; Usage: mv current/path/filename destination/path/filename |
cp | Copy (also used to rename files if you keep them in their current path), keeps a copy in the current path; Usage: cp current/path/filename destination/path/filename |
1.5-2μL | 0.8μL |
1μL | 0.4μL |
1μL | 0.4μL |
0.1μL | 0.08μL |
1μL | 1μL |