WangLab:Inhibitors
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Inhibitors
- Jasplakinolide (molecular probes, J-7473): 4 uM 15 min, or 2 M 15 min, 25nM 4h or 50nM 2h give good morphorlogy
- ML7: blocking MLCK, 5mM stock, 30 M working concentration.
For HUVECs
- Cyto D: 0.2 uM, 1 hr
- Taxol: 0.5 uM, 2 hr
- Noco: 1 uM, 1 hr
| Targeting Molecule | Inhibitors | Concentration (uM) | Duration (min) |
| Src | PP2 | 10 | 1 hr |
| Src | PP1 | 10 | 1 hr |
| Src | SU6656 | 10 | 1 hr |
| RTK | Saurumine | 50 | 1 hr |
| MAPK | PD98… | 10 | 1 hr |
| FAK | AG82… | 20 | 1 hr |
| FAK | NVP-TAE226 | 1 uM | 4 hr? |
| FLK-1 (VEGFR-2) | SU4819 | 5 uM | 1 hr |
| MicroTubules | Colchicine | 1 | 1 hr |
| MicroTubules | Nocodazole | 1 | 1 hr |
| MicroTubules | Paclitaxel (taxol; stablizing) | 1 uM | 2 hr |
| Actin filaments | Cyto D | 1 | 1 hr |
| Actin filaments | Latrunculin B (sequester G-monomer) | 0.5 uM | 1 hr |
| Actin filaments | Jasplakinolite (stabilizing) | 100 nM | 1 hr |
| PI3K | Wortmannin | 1 | 1 hr |
| Myosin ATPase | BDM | 10 mM | 1 hr |
| Myosin II | Blebbistatin | 10 uM | 1 hr |
| ROCK | Y27… | 10 | 1 hr |
| MLCK | ML-7; ML-9 | 10 or 5; 7.5 | 1 hr |
| PI3K | LY 29… | 50 | 1 hr |
| apoptosis | Pancaspase inhibitor zVAD-fmk | 10 uM | |
| de novo protein synthesis | cycloheximide | 25 ug/ml |
| Targeting Molecule | Stimuli | Concentration (uM) | Duration (min) |
| RhoA | LPA | 200 ng/ml | 30 min |
| Rac | PDGF | 25 ng/ml | 30 min |
| Cdc42 | bradykinin | 100 nM | 3 min |
| integrins a5b1, avb3, avb5 | Fibronectin | 10 ug/ml | 1 hr |
| integrins avb3 | Fibronogen, Vitronectin | ||
| integrins a1b1, a2b1 | collagen | ||
| integrins a1b1, a2b1, a3b1 and a6b1 | laminin |
serum starved for 16 hr. Cells were either treated with LPA (200 ng/ml−1) for 30 min (Sigma, Poole, UK), PDGF (25 ng/ml−1) for 30 min (TCS Biologicals, Botolph Claydon, UK), or bradykinin (100 nM) for 3 min (Sigma) or were plated on fibronectin (BD Biosciences) (10 μg/ml) for 1 hr.
