Waldminghaus:Back Door/PlasmidCollection: Difference between revisions
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==Labelling== | ==Labelling== | ||
All plasmids When you start in the lab you get a number | All names of plasmids constructed in the Waldminghaus lab consist of pMA (for plasmid from MArburg) and a number. When you start in the lab you get a number-range (1-81, 82-162, 163-243, 244-405, 406-567, 568-729,...) to use before you get the next if needed. Label the box accordingly (pMA1-81, ...)on the top and the sides. | ||
Each tube that is put into the collection needs the following information on the tube: | Each tube that is put into the collection needs the following information on the tube: | ||
On the cap: | On the cap: | ||
* ''' | * '''Name''' - pMA1, pMA804, puc57,... | ||
On the side: | On the side: | ||
* ''' | * '''Name''' - as above | ||
* '''Antibiotic resistence''' - For example amp, kan, cam, tet,... | * '''Antibiotic resistence''' - For example amp, kan, cam, tet,... | ||
* ''' | * '''Concentration''' - in ng/µl | ||
== | ==Plasmid list== | ||
As much information as possible should be stored in the | As much information as possible should be stored in the plasmid-list which can be found in the transfer folder on the SYNMIKRO-server.''' The list and the collection should always be consistent and updated!''' Please also keep a version of the strain list in your private folder. |
Revision as of 07:38, 7 January 2013
General
The plasmid collection consists of all plasmids which are constructed in the group or that we get from somewhere. All plasmids are stored in freezer I in the Waldminghaus lab (min. 100 ng and 10µl). The plasmid collection works as a reference library and not for daily use of your favourite plasmid. It should only contain one version of each plasmid (not clone number 1-13 of some insert cloning).
Labelling
All names of plasmids constructed in the Waldminghaus lab consist of pMA (for plasmid from MArburg) and a number. When you start in the lab you get a number-range (1-81, 82-162, 163-243, 244-405, 406-567, 568-729,...) to use before you get the next if needed. Label the box accordingly (pMA1-81, ...)on the top and the sides.
Each tube that is put into the collection needs the following information on the tube:
On the cap:
- Name - pMA1, pMA804, puc57,...
On the side:
- Name - as above
- Antibiotic resistence - For example amp, kan, cam, tet,...
- Concentration - in ng/µl
Plasmid list
As much information as possible should be stored in the plasmid-list which can be found in the transfer folder on the SYNMIKRO-server. The list and the collection should always be consistent and updated! Please also keep a version of the strain list in your private folder.