Van Oudenaarden Lab:F31E3.1: Difference between revisions
(New page: Progress: Probes designed: (2-8-2010 - Ni Ji) Probes coupled: 2-25-2010 Probes purified: 2-27-2010 Probes tested: Dyes available: Cy-5 >K02B12.1 (ceh-20 spliced coding region) ATGG...) |
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Dyes available: Cy-5 | Dyes available: Cy-5 | ||
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>K02B12.1 (ceh-20 spliced coding region) | >K02B12.1 (ceh-20 spliced coding region) | ||
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CCTACAATCC TATGAATATT CCCGGACAAG ACACATTGCA TATGGGAATG CCACCCTTTG ATTTATCGGT ATATAATCCA | CCTACAATCC TATGAATATT CCCGGACAAG ACACATTGCA TATGGGAATG CCACCCTTTG ATTTATCGGT ATATAATCCA | ||
CAATTGATGG CCGCAGCTCA ATACCAACAA CAAATGGACA ATGCTGATAA AAATTCATAA | CAATTGATGG CCGCAGCTCA ATACCAACAA CAAATGGACA ATGCTGATAA AAATTCATAA | ||
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Name of probe set: ceh-20 | Name of probe set: ceh-20 | ||
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gctgggtgtgtccccaccat, Probe_1, 1, 1, 65 | gctgggtgtgtccccaccat, Probe_1, 1, 1, 65 | ||
gtctaaaagctcgctgagat, Probe_2, 2, 23, 45 | gtctaaaagctcgctgagat, Probe_2, 2, 23, 45 | ||
gctcatttattttcaagacg, Probe_3, 3, 45, 35 | gctcatttattttcaagacg, Probe_3, 3, 45, 35 | ||
ctgtcattatcatccaaagt, Probe_4, 4, 67, 35 | ctgtcattatcatccaaagt, Probe_4, 4, 67, 35 | ||
ttgtagctcttgtttttttg, Probe_5, 5, 89, 30 | ttgtagctcttgtttttttg, Probe_5, 5, 89, 30 | ||
gggcttgacgcatgggatga, Probe_6, 6, 111, 60 | gggcttgacgcatgggatga, Probe_6, 6, 111, 60 | ||
gtttcacagagaacatcgaa, Probe_7, 7, 133, 40 | gtttcacagagaacatcgaa, Probe_7, 7, 133, 40 | ||
ctgtcagtactgttttttct, Probe_8, 8, 156, 35 | ctgtcagtactgttttttct, Probe_8, 8, 156, 35 | ||
gtttcgtccacttgattacg, Probe_9, 9, 178, 45 | gtttcgtccacttgattacg, Probe_9, 9, 178, 45 | ||
cattaattgaggatcttctg, Probe_10, 10, 200, 35 | cattaattgaggatcttctg, Probe_10, 10, 200, 35 | ||
ccactaacatattatcaagt, Probe_11, 11, 222, 30 | ccactaacatattatcaagt, Probe_11, 11, 222, 30 | ||
tctggtcctgcaacgccttc, Probe_12, 12, 244, 60 | tctggtcctgcaacgccttc, Probe_12, 12, 244, 60 | ||
acttcctaatgaacctcctt, Probe_13, 13, 266, 40 | acttcctaatgaacctcctt, Probe_13, 13, 266, 40 | ||
cctgatcacctccacttgca, Probe_14, 14, 288, 55 | cctgatcacctccacttgca, Probe_14, 14, 288, 55 | ||
tgaagcttttgtctataatc, Probe_15, 15, 310, 30 | tgaagcttttgtctataatc, Probe_15, 15, 310, 30 | ||
attgtacaaaactcgaatct, Probe_16, 16, 332, 30 | attgtacaaaactcgaatct, Probe_16, 16, 332, 30 | ||
cctcatactttctcagctct, Probe_17, 17, 354, 45 | cctcatactttctcagctct, Probe_17, 17, 354, 45 | ||
tgcgtgaattcgttacaagc, Probe_18, 18, 376, 45 | tgcgtgaattcgttacaagc, Probe_18, 18, 376, 45 | ||
cttcagcagagacctcacgt, Probe_19, 19, 398, 55 | cttcagcagagacctcacgt, Probe_19, 19, 398, 55 | ||
ttgggcgaacttgtgattga, Probe_20, 20, 420, 45 | ttgggcgaacttgtgattga, Probe_20, 20, 420, 45 | ||
cgttcgatttccttatgtgc, Probe_21, 21, 442, 45 | cgttcgatttccttatgtgc, Probe_21, 21, 442, 45 | ||
tcgttggattatataaacca, Probe_22, 22, 464, 30 | tcgttggattatataaacca, Probe_22, 22, 464, 30 | ||
gaacttgaattccattgaat, Probe_23, 23, 486, 30 | gaacttgaattccattgaat, Probe_23, 23, 486, 30 | ||
tcacatgtggattgtttgag, Probe_24, 24, 508, 40 | tcacatgtggattgtttgag, Probe_24, 24, 508, 40 | ||
gcttctgaggatcatgacgg, Probe_25, 25, 530, 55 | gcttctgaggatcatgacgg, Probe_25, 25, 530, 55 | ||
tacggcgggcatcaaggaac, Probe_26, 26, 552, 60 | tacggcgggcatcaaggaac, Probe_26, 26, 552, 60 | ||
tgtttcgaaaagtttcgccg, Probe_27, 27, 574, 45 | tgtttcgaaaagtttcgccg, Probe_27, 27, 574, 45 | ||
ttcattaagcacttctgtcg, Probe_28, 28, 596, 40 | ttcattaagcacttctgtcg, Probe_28, 28, 596, 40 | ||
ttgagaggtgtccatagaaa, Probe_29, 29, 618, 40 | ttgagaggtgtccatagaaa, Probe_29, 29, 618, 40 | ||
gcttcttctgatgggtatgg, Probe_30, 30, 640, 50 | gcttcttctgatgggtatgg, Probe_30, 30, 640, 50 | ||
ctgccttgcgagatcttctt, Probe_31, 31, 662, 50 | ctgccttgcgagatcttctt, Probe_31, 31, 662, 50 | ||
cctgagatactgtaatattg, Probe_32, 32, 684, 35 | cctgagatactgtaatattg, Probe_32, 32, 684, 35 | ||
ttatttccaaaccaattgga, Probe_33, 33, 706, 30 | ttatttccaaaccaattgga, Probe_33, 33, 706, 30 | ||
atttttcttgtagcgaattc, Probe_34, 34, 728, 30 | atttttcttgtagcgaattc, Probe_34, 34, 728, 30 | ||
cttcctcttgagcttttgcc, Probe_35, 35, 750, 50 | cttcctcttgagcttttgcc, Probe_35, 35, 750, 50 | ||
tttttggcagcatacatact, Probe_36, 36, 772, 35 | tttttggcagcatacatact, Probe_36, 36, 772, 35 | ||
tcctaatgttacatgagcat, Probe_37, 37, 794, 35 | tcctaatgttacatgagcat, Probe_37, 37, 794, 35 | ||
cgtatggatttccagccata, Probe_38, 38, 816, 45 | cgtatggatttccagccata, Probe_38, 38, 816, 45 | ||
gctgcagcaccaggaagcat, Probe_39, 39, 838, 60 | gctgcagcaccaggaagcat, Probe_39, 39, 838, 60 | ||
gggatttaataggccagcag, Probe_40, 40, 860, 50 | gggatttaataggccagcag, Probe_40, 40, 860, 50 | ||
cgggaatattcataggattg, Probe_41, 41, 882, 40 | cgggaatattcataggattg, Probe_41, 41, 882, 40 | ||
cccatatgcaatgtgtcttg, Probe_42, 42, 904, 45 | cccatatgcaatgtgtcttg, Probe_42, 42, 904, 45 | ||
cgataaatcaaagggtggca, Probe_43, 43, 926, 45 | cgataaatcaaagggtggca, Probe_43, 43, 926, 45 | ||
gccatcaattgtggattata, Probe_44, 44, 949, 35 | gccatcaattgtggattata, Probe_44, 44, 949, 35 | ||
ttgttgttggtattgagctg, Probe_45, 45, 971, 40 | ttgttgttggtattgagctg, Probe_45, 45, 971, 40 | ||
aatttttatcagcattgtcc, Probe_46, 46, 993, 30 | aatttttatcagcattgtcc, Probe_46, 46, 993, 30 | ||
Revision as of 18:28, 1 March 2010
Progress:
Probes designed: (2-8-2010 - Ni Ji)
Probes coupled: 2-25-2010
Probes purified: 2-27-2010
Probes tested:
Dyes available: Cy-5
>K02B12.1 (ceh-20 spliced coding region)
ATGGTGG GGACACACCC AGCCAATCTC AGCGAGCTTT TAGACGCCGT CTTGAAAATA AATGAGCAAA CTTTGGATGA TAATGACAGT GCAAAAAAAC AAGAGCTACA ATGTCATCCC ATGCGTCAAG CCCTATTCGA TGTTCTCTGT GAAACAAAAG AAAAAACAGT ACTGACAGTT CGTAATCAAG TGGACGAAAC TCCAGAAGAT CCTCAATTAA TGCGACTTGA TAATATGTTA GTGGCGGAAG GCGTTGCAGG ACCAGATAAA GGAGGTTCAT TAGGAAGTGA TGCAAGTGGA GGTGATCAGG CTGATTATAG ACAAAAGCTT CACCAGATTC GAGTTTTGTA CAATGAAGAG CTGAGAAAGT ATGAGGAGGC TTGTAACGAA TTCACGCAAC ACGTGAGGTC TCTGCTGAAG GATCAATCAC AAGTTCGCCC AATAGCACAT AAGGAAATCG AACGAATGGT TTATATAATC CAACGAAAAT TCAATGGAAT TCAAGTTCAA CTCAAACAAT CCACATGTGA GGCCGTCATG ATCCTCAGAA GCAGGTTCCT TGATGCCCGC CGTAAACGGC GAAACTTTTC GAAACAAGCG ACAGAAGTGC TTAATGAATA TTTCTATGGA CACCTCTCAA ATCCATACCC ATCAGAAGAA GCAAAAGAAG ATCTCGCAAG GCAGTGCAAT ATTACAGTAT CTCAGGTTTC CAATTGGTTT GGAAATAAAC GAATTCGCTA CAAGAAAAAT ATGGCAAAAG CTCAAGAGGA AGCCAGTATG TATGCTGCCA AAAAGAATGC TCATGTAACA TTAGGAGGTA TGGCTGGAAA TCCATACGGA ATGCTTCCTG GTGCTGCAGC CGCTGCTGGC CTATTAAATC CCTACAATCC TATGAATATT CCCGGACAAG ACACATTGCA TATGGGAATG CCACCCTTTG ATTTATCGGT ATATAATCCA CAATTGATGG CCGCAGCTCA ATACCAACAA CAAATGGACA ATGCTGATAA AAATTCATAA
Name of probe set: ceh-20
Probes designed on 8 Feb 10
46 probes designed for target of length 1017
Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC
gctgggtgtgtccccaccat, Probe_1, 1, 1, 65
gtctaaaagctcgctgagat, Probe_2, 2, 23, 45
gctcatttattttcaagacg, Probe_3, 3, 45, 35
ctgtcattatcatccaaagt, Probe_4, 4, 67, 35
ttgtagctcttgtttttttg, Probe_5, 5, 89, 30
gggcttgacgcatgggatga, Probe_6, 6, 111, 60
gtttcacagagaacatcgaa, Probe_7, 7, 133, 40
ctgtcagtactgttttttct, Probe_8, 8, 156, 35
gtttcgtccacttgattacg, Probe_9, 9, 178, 45
cattaattgaggatcttctg, Probe_10, 10, 200, 35
ccactaacatattatcaagt, Probe_11, 11, 222, 30
tctggtcctgcaacgccttc, Probe_12, 12, 244, 60
acttcctaatgaacctcctt, Probe_13, 13, 266, 40
cctgatcacctccacttgca, Probe_14, 14, 288, 55
tgaagcttttgtctataatc, Probe_15, 15, 310, 30
attgtacaaaactcgaatct, Probe_16, 16, 332, 30
cctcatactttctcagctct, Probe_17, 17, 354, 45
tgcgtgaattcgttacaagc, Probe_18, 18, 376, 45
cttcagcagagacctcacgt, Probe_19, 19, 398, 55
ttgggcgaacttgtgattga, Probe_20, 20, 420, 45
cgttcgatttccttatgtgc, Probe_21, 21, 442, 45
tcgttggattatataaacca, Probe_22, 22, 464, 30
gaacttgaattccattgaat, Probe_23, 23, 486, 30
tcacatgtggattgtttgag, Probe_24, 24, 508, 40
gcttctgaggatcatgacgg, Probe_25, 25, 530, 55
tacggcgggcatcaaggaac, Probe_26, 26, 552, 60
tgtttcgaaaagtttcgccg, Probe_27, 27, 574, 45
ttcattaagcacttctgtcg, Probe_28, 28, 596, 40
ttgagaggtgtccatagaaa, Probe_29, 29, 618, 40
gcttcttctgatgggtatgg, Probe_30, 30, 640, 50
ctgccttgcgagatcttctt, Probe_31, 31, 662, 50
cctgagatactgtaatattg, Probe_32, 32, 684, 35
ttatttccaaaccaattgga, Probe_33, 33, 706, 30
atttttcttgtagcgaattc, Probe_34, 34, 728, 30
cttcctcttgagcttttgcc, Probe_35, 35, 750, 50
tttttggcagcatacatact, Probe_36, 36, 772, 35
tcctaatgttacatgagcat, Probe_37, 37, 794, 35
cgtatggatttccagccata, Probe_38, 38, 816, 45
gctgcagcaccaggaagcat, Probe_39, 39, 838, 60
gggatttaataggccagcag, Probe_40, 40, 860, 50
cgggaatattcataggattg, Probe_41, 41, 882, 40
cccatatgcaatgtgtcttg, Probe_42, 42, 904, 45
cgataaatcaaagggtggca, Probe_43, 43, 926, 45
gccatcaattgtggattata, Probe_44, 44, 949, 35
ttgttgttggtattgagctg, Probe_45, 45, 971, 40
aatttttatcagcattgtcc, Probe_46, 46, 993, 30
