Van Oudenaarden Lab:C28A5.4

Progress:

Probes designed: (03-01-2010 - Alexander van Oudenaarden)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:

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>C28A5.4 (ceh-43 spliced coding region)

atggacccatcaaaaggattcgaatatgttgctggagattattatcagacatctggagtagctccacccacaagcaacgg agctggatctaacgtttccccttatttcccctaccatgcttatccgacgtcatcaacgaatggagcaactggtggttcta tgtatggtacaccacagcaaacgagtgcctatgcaatgtatcctcctgggccaggaagctcacctgaagaagcttttccc gagcacacaactacaaaaatcgtggaaggatgtgaggcgaagtacaacgtgaaaggaaagaaaatgaggaaaccacggac aatttacaactcatcacaactgcaaatgcttcagaaaaaattccaaaaaacacaatatctggcgcttccggatcgagcag cattggcacatgaattgggattatcacaaactcaggtgaaaatctggttccaaaatcggcgttcgaagcagaagaaacag aagggtggaagttcggatcatgcatcagatgaagaagatgatgatacggaagagtcaaagcctgaaagtccaccgatggg agaatcggttatgattcaggagagcagtgagccaagaacacttgtatctagttcaattaaaactgaaatgaaagaggaat atccaccgatgacactcaacgagcaatatgcttctccttacctctacggatccgacttttcaacgattctcccaccgtca cagggtttccccaacaatgcgctctacaatacggcaggcgcttatcctagcattgactacacaaatggagtataccagaa tactctttacaagtatgtctga

ggatataatatttcctttttc ttgactgtcagttaatttaaaaaattcttttttacaaggctcttgtagtaccttgctaaaatatgttcacttttgaataa gctgtcagcctagaaaatattgattttctcgatgatttgtgtttttttctttccaaacttttctatggtctcatggtttt tgatatataaatcgatattgaacgttt

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Name of probe set: ceh-43

Probes designed on Mon, 01 Mar 10 13:45:09 -0700

46 probes designed for target of length 1030 (target too short for 48 probes)

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

aatccttttgatgggtccat, ceh-43_1, 1, 1, 40

ataatctccagcaacatatt, ceh-43_2, 2, 23, 30

gagctactccagatgtctga, ceh-43_3, 3, 45, 50

ccagctccgttgcttgtggg, ceh-43_4, 4, 67, 65

gaaataaggggaaacgttag, ceh-43_5, 5, 89, 40

acgtcggataagcatggtag, ceh-43_6, 6, 111, 50

ccagttgctccattcgttga, ceh-43_7, 7, 133, 50

tggtgtaccatacatagaac, ceh-43_8, 8, 155, 40

ttgcataggcactcgtttgc, ceh-43_9, 9, 177, 50

cttcctggcccaggaggata, ceh-43_10, 10, 199, 60

gggaaaagcttcttcaggtg, ceh-43_11, 11, 221, 50

cgatttttgtagttgtgtgc, ceh-43_12, 12, 243, 40

tacttcgcctcacatccttc, ceh-43_13, 13, 265, 50

cattttctttcctttcacgt, ceh-43_14, 14, 287, 35

tgtaaattgtccgtggtttc, ceh-43_15, 15, 309, 40

agcatttgcagttgtgatga, ceh-43_16, 16, 331, 40

tgttttttggaattttttct, ceh-43_17, 17, 353, 20

gatccggaagcgccagatat, ceh-43_18, 18, 375, 55

aattcatgtgccaatgctgc, ceh-43_19, 19, 397, 45

cacctgagtttgtgataatc, ceh-43_20, 20, 419, 40

gccgattttggaaccagatt, ceh-43_21, 21, 441, 45

cttctgtttcttctgcttcg, ceh-43_22, 22, 464, 45

atgcatgatccgaacttcca, ceh-43_23, 23, 486, 45

gtatcatcatcttcttcatc, ceh-43_24, 24, 508, 35

actttcaggctttgactctt, ceh-43_25, 25, 530, 40

taaccgattctcccatcggt, ceh-43_26, 26, 552, 50

ggctcactgctctcctgaat, ceh-43_27, 27, 574, 55

tgaactagatacaagtgttc, ceh-43_28, 28, 596, 35

cctctttcatttcagtttta, ceh-43_29, 29, 618, 30

ttgagtgtcatcggtggata, ceh-43_30, 30, 640, 45

gtaaggagaagcatattgct, ceh-43_31, 31, 662, 40

ttgaaaagtcggatccgtag, ceh-43_32, 32, 684, 45

ccctgtgacggtgggagaat, ceh-43_33, 33, 706, 60

gtagagcgcattgttgggga, ceh-43_34, 34, 728, 55

taggataagcgcctgccgta, ceh-43_35, 35, 750, 55

actccatttgtgtagtcaat, ceh-43_36, 36, 772, 35

cttgtaaagagtattctggt, ceh-43_37, 37, 794, 35

gaaatattatatcctcagac, ceh-43_38, 38, 817, 30

ttaactgacagtcaagaaaa, ceh-43_39, 39, 839, 30

caagagccttgtaaaaaaga, ceh-43_40, 40, 869, 35

gtgaacatattttagcaagg, ceh-43_41, 41, 894, 35

ctaggctgacagcttattca, ceh-43_42, 42, 917, 45

tcatcgagaaaatcaatatt, ceh-43_43, 43, 939, 25

gtttggaaagaaaaaaacac, ceh-43_44, 44, 962, 30

caaaaaccatgagaccatag, ceh-43_45, 45, 986, 40

cgttcaatatcgatttatat, ceh-43_46, 46, 1008, 25