Van Oudenaarden Lab:C02B8.4: Difference between revisions

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>C02B8.4 (hlh-8 spliced coding region)
atggtggctcgtagaaaaggagaacgcgtggttcgaaagaatgaagtggaaaatgtgcagcagagagcatgtgccaacag
acgggaacgtcaaaggaccaaggaactcaatgacgctttcacacttttgcgtaaactgatcccgtcgatgccctcagaca
agatgagcaagatccacacactacgcattgcaaccgattacatatccttccttgacgagatgcaaaaaaatggatgcaaa
ctgtatggccattcgatcttcgacgaaaaacgtggatacaatttacagtcggctttcaatatgtggaggggtaacaatgg
atacacaccaattgctggaccttcacagttgcctccactacaatctgcacatattccccctcctgctccatccagcattc
ctccacactgtctgatgccccagccttggtatcagacttgtccgccgccaaaacaagaatttcatgaactgtgtcccatc
tcaactccaaatcccaattcaaatccaaatcaactcactccaattcactggcaataa
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Name of probe set: hlh-8
Probes designed on Mon, 01 Mar 10 14:27:57 -0700
24 probes designed for target of length 537 (target too short for 48 probes)
Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC
ccttttctacgagccaccat, hlh-8_1, 1, 1, 50
attctttcgaaccacgcgtt, hlh-8_2, 2, 23, 45
tctgctgcacattttccact, hlh-8_3, 3, 45, 45
tcccgtctgttggcacatgc, hlh-8_4, 4, 67, 60
gagttccttggtcctttgac, hlh-8_5, 5, 89, 50
gcaaaagtgtgaaagcgtca, hlh-8_6, 6, 111, 45
ggcatcgacgggatcagttt, hlh-8_7, 7, 133, 55
gatcttgctcatcttgtctg, hlh-8_8, 8, 155, 45
tcggttgcaatgcgtagtgt, hlh-8_9, 9, 178, 50
ctcgtcaaggaaggatatgt, hlh-8_10, 10, 200, 45
gtttgcatccatttttttgc, hlh-8_11, 11, 222, 35
tcgaagatcgaatggccata, hlh-8_12, 12, 244, 45
gactgtaaattgtatccacg, hlh-8_13, 13, 271, 40
acccctccacatattgaaag, hlh-8_14, 14, 293, 45
caattggtgtgtatccattg, hlh-8_15, 15, 315, 40
ggaggcaactgtgaaggtcc, hlh-8_16, 16, 337, 60
gggaatatgtgcagattgta, hlh-8_17, 17, 359, 40
gaatgctggatggagcagga, hlh-8_18, 18, 381, 55
tggggcatcagacagtgtgg, hlh-8_19, 19, 403, 60
cggacaagtctgataccaag, hlh-8_20, 20, 425, 50
catgaaattcttgttttggc, hlh-8_21, 21, 447, 35
tggagttgagatgggacaca, hlh-8_22, 22, 470, 50
gatttggatttgaattggga, hlh-8_23, 23, 492, 35
ttgccagtgaattggagtga, hlh-8_24, 24, 515, 45

Latest revision as of 14:28, 1 March 2010

Progress:

Probes designed: (03-01-2010 - Alexander van Oudenaarden)

Probes coupled:

Probes purified:

Probes tested:

Dyes available:


>C02B8.4 (hlh-8 spliced coding region)

atggtggctcgtagaaaaggagaacgcgtggttcgaaagaatgaagtggaaaatgtgcagcagagagcatgtgccaacag acgggaacgtcaaaggaccaaggaactcaatgacgctttcacacttttgcgtaaactgatcccgtcgatgccctcagaca agatgagcaagatccacacactacgcattgcaaccgattacatatccttccttgacgagatgcaaaaaaatggatgcaaa ctgtatggccattcgatcttcgacgaaaaacgtggatacaatttacagtcggctttcaatatgtggaggggtaacaatgg atacacaccaattgctggaccttcacagttgcctccactacaatctgcacatattccccctcctgctccatccagcattc ctccacactgtctgatgccccagccttggtatcagacttgtccgccgccaaaacaagaatttcatgaactgtgtcccatc tcaactccaaatcccaattcaaatccaaatcaactcactccaattcactggcaataa


Name of probe set: hlh-8

Probes designed on Mon, 01 Mar 10 14:27:57 -0700

24 probes designed for target of length 537 (target too short for 48 probes)

Probe (5'->3'), Probe name, Probe number, Probe position, Percent GC

ccttttctacgagccaccat, hlh-8_1, 1, 1, 50

attctttcgaaccacgcgtt, hlh-8_2, 2, 23, 45

tctgctgcacattttccact, hlh-8_3, 3, 45, 45

tcccgtctgttggcacatgc, hlh-8_4, 4, 67, 60

gagttccttggtcctttgac, hlh-8_5, 5, 89, 50

gcaaaagtgtgaaagcgtca, hlh-8_6, 6, 111, 45

ggcatcgacgggatcagttt, hlh-8_7, 7, 133, 55

gatcttgctcatcttgtctg, hlh-8_8, 8, 155, 45

tcggttgcaatgcgtagtgt, hlh-8_9, 9, 178, 50

ctcgtcaaggaaggatatgt, hlh-8_10, 10, 200, 45

gtttgcatccatttttttgc, hlh-8_11, 11, 222, 35

tcgaagatcgaatggccata, hlh-8_12, 12, 244, 45

gactgtaaattgtatccacg, hlh-8_13, 13, 271, 40

acccctccacatattgaaag, hlh-8_14, 14, 293, 45

caattggtgtgtatccattg, hlh-8_15, 15, 315, 40

ggaggcaactgtgaaggtcc, hlh-8_16, 16, 337, 60

gggaatatgtgcagattgta, hlh-8_17, 17, 359, 40

gaatgctggatggagcagga, hlh-8_18, 18, 381, 55

tggggcatcagacagtgtgg, hlh-8_19, 19, 403, 60

cggacaagtctgataccaag, hlh-8_20, 20, 425, 50

catgaaattcttgttttggc, hlh-8_21, 21, 447, 35

tggagttgagatgggacaca, hlh-8_22, 22, 470, 50

gatttggatttgaattggga, hlh-8_23, 23, 492, 35

ttgccagtgaattggagtga, hlh-8_24, 24, 515, 45