http://openwetware.org/index.php?title=Using_the_NanoDrop&feed=atom&action=historyUsing the NanoDrop - Revision history2013-06-19T11:42:25ZRevision history for this page on the wikiMediaWiki 1.13.2http://openwetware.org/index.php?title=Using_the_NanoDrop&diff=605606&oldid=prevAlex Zorychta: New page: ==Overview== Detailed below is the procedure to use the NanoDrop machine to quantify amount of nucleic acid, specifically as prepared from [this protocol]. ==Materials== * Sterile Water...2012-06-04T16:35:35Z<p>New page: ==Overview== Detailed below is the procedure to use the NanoDrop machine to quantify amount of nucleic acid, specifically as prepared from [this protocol]. ==Materials== * Sterile Water...</p>
<p><b>New page</b></p><div>==Overview==<br />
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Detailed below is the procedure to use the NanoDrop machine to quantify amount of nucleic acid, specifically as prepared from [this protocol].<br />
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==Materials==<br />
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* Sterile Water<br />
* Buffer without sample<br />
* Sample in buffer<br />
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==Procedure==<br />
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'''Be very careful using the machine, the other lab is very strict about it and misuse may cause us to lose our privilege of using it.'''<br />
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* Open up the NanoDrop application.<br />
* Choose "Nucleic Acid" on the GUI.<br />
* Aliquot 2 μL of sterile water on the sample space of the machine and then close it.<br />
* Click "OK." Make sure it clicks as it reads the sample.<br />
* Wipe the sample space with a Kimwipe.<br />
* Next, put in 2 μL of the buffer ''without the sample'' and click "Blank." Wipe it off with a Kimwipe. ''This is our control measurement.''<br />
* Next, put in 2 μL of your sample. Click "Measure." Then clean the sample space with a Kimwipe.<br />
* Lastly, put on 2 μL of sterile water again, press it down, then move it back up again, and wipe it with a Kimwipe.<br />
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Name the file, click "Show Report," and "Save Window" into the shared CVRC folder to access it on our own computers.<br />
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==Notes==<br />
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!<br />
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==References==<br />
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==Contact==<br />
*Who has experience with this protocol?<br />
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or instead, [[Talk:{{PAGENAME}}|discuss this protocol]]. <br />
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[[Category:DNA]]<br />
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--></div>Alex Zorychta