User talk:Rebeca Rodriguez/Notebook/581/2014/10/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Data== | ==Data== | ||
===Fluorescents=== | |||
0.0120g of R6G in 10mL needed to make a 2500uM stock solution. This solution was used to make 2.0uM. The solution was diluted further (1:5), to create a 500uM stock solution in order to make the dilutions for 0.1, 0.5, 1.0, and 1.5. | |||
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| align="center" style="background:#f0f0f0;"|'''Stock Concentrations (uM)''' | |||
| align="center" style="background:#f0f0f0;"|'''Amount of Stock Solution Needed (mL)''' | |||
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| 0.1||0.001 | |||
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| 0.5||0.005 | |||
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| 1||0.01 | |||
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| 1.5||0.015 | |||
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| 2||0.004 | |||
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| align="center" style="background:#f0f0f0;"|'''Fluorescent Parameters''' | |||
| align="center" style="background:#f0f0f0;"|'''Value''' | |||
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| Exitation Wavelength||500 nm | |||
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| Range||515nm -700nm | |||
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| Slit Width||10nm | |||
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| Scan Speed||200 nm/min | |||
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==Notes== | ==Notes== |
Latest revision as of 00:21, 27 September 2017
Biomaterials Design Lab | Main project page | ||||||||||||||||||||||
ObjectiveTwo groups will be working on determining fluorophore concentration. (Alicia/Madeleine and Becky/Eleni/Melvin) The other two groups will be working on diffusion. (Jake/James/Mary and Andrew/Michael/Tami). NOTE: The groups working on fluorophores will need to space to the groups working on diffusion as their research is very time sensitive. DescriptionFluorescence
Diffusion
DataFluorescents0.0120g of R6G in 10mL needed to make a 2500uM stock solution. This solution was used to make 2.0uM. The solution was diluted further (1:5), to create a 500uM stock solution in order to make the dilutions for 0.1, 0.5, 1.0, and 1.5.
NotesThis area is for any observations or conclusions that you would like to note.
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