User talk:Isabelle Rocca/Notebook/Biology 210 at AU: Difference between revisions

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== Hay Infusion Culture Lab ==
'''3/21/2015'''
Your zebrafis entry is a little brief. In the methods, what was the concentration of rhodamine that you used? What stage were the embryos when you started? On day 4, did you keep track of how many embryos had hatched? Numbers are always better! It is fine if you did not see differences between the experimental fish and the controls, but you need to provide at least some quantitative data. Did you measure the length of the day 7 fish that you preserved? Ideally you would be able to say something like "Three fish from each group were measured. The mean length of the control fish was 1.0± .3 mm and the length of the experimental fish was 1.1 ± .4 mm, which was not statistically different." You need more details rather than generalizations. LS


'''1/25/15'''
 
'''Purpose:'''
'''3/6/2015'''
In this lab, we are creating a culture in order to examine the forms of life that are present around the school. Each lab group was given a different transect of a different wildlife area around the school. We observed the living and non-living components that make up a biological niche. My group’s transect resembled a marshy/ swamp sort of land, because it was right by a water drain. I hypothesize that the types of organisms we will observe will be found commonly around areas of water and mossy life.
You need a little more information about your identified species. Are they tetracycline resistant, and did the colony morphology and cell shape of the identified species match what you found? LS
'''Materials:'''
 
• A wildlife transect found somewhere on the grounds of AU
'''2/22/2015'''
• Paper
 
• Pencil
Vert lab - Good job on identification and thinking about interactions. I'm glad you included bacteria and inverts in your food web, but include the birds and toad too!
• 50 ml conical tube
 
• 500 mls water
Invert lab - Great! The planaria photo is so cute. LS
• .1 g of dried milk
 
• Medium sized plastic jar
'''2/15/2015'''
'''Methods:'''
This is very good. For the lab report I would like Genus names for at least the cat tail and the flowering plant. It is unique-looking, so I think you will be able to narrow it down. Even if you can't find seeds, you can tell if an angiosperm is a monocot or dicot by looking at the leaves and flowers. Grasses are generally monocots. LS
1. Go out to assigned transect and observe the biotic and abiotic substance found there
 
2. Draw an aerial view of the transect
'''2/9/2015'''
3. Describe the location, and topography of the transect
Great! I can see your google docs now.
4. In the conical tube collect 50% soil from the area, and 50% vegetation from the area
 
5. Back in the lab, weigh out 10-12 grams of the collected soil/vegetation, and place it in the jar
'''1/26/2015'''
6. Add 500 mls of water to the jar with the sample
This is a good start. I might re-title something like "Preliminary Characterization of an Ecosystem/Niche" because you did more than start the Hay Infusion. Also don't forget to upload/link to your map. LS
7. Finally add the dried milk to the water and the sample in the jar
 
8. Put the lid on the jar and gently mix for 10 seconds
'''1/30/2015'''
9. Open the jar and place it somewhere safe in the lab
Very good. You provided thoughtful answers to the questions. Next time, use your own photos or drawings rather than those from the dichotomous key. LS
'''Data and Observations:'''
The transect of my group was on the north eastern side of campus, in the front of the Kogod building. It is supposed to have the characteristics of a marshy/ swamp land, because it is located by a water drain. Most of the plants found in the small garden were dead for the winter, but there was still green grass and moss present. Some of the biotic features that can be found in the transect are grass, moss, red cardinal flowers, cat tails, and straw plants. Some abiotic features that are there are rocks, snow, a metal sign, trash, and soil. The sample obtained for the culture consisted of soil, snow, moss, some leaves from the plants, grass, and some straw from the plant.  
'''Conclusion:'''
Back at the lab, a hay infusion culture was made using the sample from the transect. It will incubate for a week, so that next week, the living organisms that are present in the transect will be observable under the microscope. This will demonstrate what sort of organisms grow in different environments around the school.
IR

Latest revision as of 18:00, 21 March 2015

3/21/2015 Your zebrafis entry is a little brief. In the methods, what was the concentration of rhodamine that you used? What stage were the embryos when you started? On day 4, did you keep track of how many embryos had hatched? Numbers are always better! It is fine if you did not see differences between the experimental fish and the controls, but you need to provide at least some quantitative data. Did you measure the length of the day 7 fish that you preserved? Ideally you would be able to say something like "Three fish from each group were measured. The mean length of the control fish was 1.0± .3 mm and the length of the experimental fish was 1.1 ± .4 mm, which was not statistically different." You need more details rather than generalizations. LS


3/6/2015 You need a little more information about your identified species. Are they tetracycline resistant, and did the colony morphology and cell shape of the identified species match what you found? LS

2/22/2015

Vert lab - Good job on identification and thinking about interactions. I'm glad you included bacteria and inverts in your food web, but include the birds and toad too!

Invert lab - Great! The planaria photo is so cute. LS

2/15/2015 This is very good. For the lab report I would like Genus names for at least the cat tail and the flowering plant. It is unique-looking, so I think you will be able to narrow it down. Even if you can't find seeds, you can tell if an angiosperm is a monocot or dicot by looking at the leaves and flowers. Grasses are generally monocots. LS

2/9/2015 Great! I can see your google docs now.

1/26/2015 This is a good start. I might re-title something like "Preliminary Characterization of an Ecosystem/Niche" because you did more than start the Hay Infusion. Also don't forget to upload/link to your map. LS

1/30/2015 Very good. You provided thoughtful answers to the questions. Next time, use your own photos or drawings rather than those from the dichotomous key. LS

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