User talk:Darek Kedra/sandbox 29: Difference between revisions
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Revision as of 07:51, 9 September 2014
EMBO Tunis 2014
From sequencing data to knowledge
00 Programs used
- [ http://www.usadellab.org/cms/index.php?page=trimmomatic Trimmomatic ] ver 0.32
- [ http://picard.sourceforge.net/ Picard ] ver 1.119
NGS file formats
- FASTQ
- SAM
- BAM,
- VCF,
- GTF/GFF
- BED
pre-processing FASTQ files =
Mapping Illumina reads with LAST (possibly also with BWA/Stampy: check with G.Benson) Quick and dirty genome 2 genome comparison using LAST Viewing mapping results with IGV SNP discovery Quantifications of mapped reads Finding gene ends by mapping post-splice leader and polyA sequences Mapping Illumina reads using LAST Comparing 2-3 Leishmania genomes Viewing mappings and SNPs Gene quantifications (DNA & RNA levels)
