User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/04/15: Difference between revisions

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==Goals==
==Goals==
#Determine the affect of functional nanoparticles being incubated at 37C for 6 days
 
#This is what we were going to do if are stuff from last Wednesday worked but it did not
* Test HRP pellet
* Spin myoglobin and HB to see if it precipitates
* UV VIS myoglobin and Hb
**This will determine if the myoglobin or HB breaks apart after spinning
<br>
<br>
<br>
<br>
#Today we spun down are nanoparticles and are determining if HRP pellets have activity
#we will then compare kinetics between the proteins


==Procedure==
==Procedure==
- If the nanoparticles were found to functional on April 9th we put them in the 37C incubator for 6 days
 
*use centrifuge to spin down proteins
*use uv-vis
 
==Notes==
 
*nothing precipitated out after we spun down the myoglobin and the hemoglobin
*the hemoglobin and myoglobin both showed activity in the solutions
<br>
<br>
- Using Ocean Optics we will determine the activity of the nanoparticles
<br>
<br>
- This will allow us to infer about the effectiveness of the nanoparticles if they were eveer injected into humans
*from the data we can see that the gamma method appears to be too concentrated to adequately study it
 
==Data==
[[Image:Screen Shot 2014-04-16 at 5.46.16 PM.png]]
 
[[Image:Screen Shot 2014-04-16 at 5.46.24 PM.png]]
 
[[Image:Screen Shot 2014-04-16 at 5.47.09 PM.png]]
 
[[Image:Screen Shot 2014-04-16 at 5.47.02 PM.png]]
 
[[Image:Screen Shot 2014-04-16 at 5.46.34 PM.png]]
 
[[Image:Screen Shot 2014-04-16 at 5.46.45 PM.png]]
 
[[Image:Screen Shot 2014-04-16 at 5.46.53 PM.png]]


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Revision as of 14:11, 22 April 2014

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April 15th

Goals

  • Test HRP pellet
  • Spin myoglobin and HB to see if it precipitates
  • UV VIS myoglobin and Hb
    • This will determine if the myoglobin or HB breaks apart after spinning



Procedure

  • use centrifuge to spin down proteins
  • use uv-vis

Notes

  • nothing precipitated out after we spun down the myoglobin and the hemoglobin
  • the hemoglobin and myoglobin both showed activity in the solutions



  • from the data we can see that the gamma method appears to be too concentrated to adequately study it

Data


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