User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/03/18: Difference between revisions
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== | ==March 18th== | ||
* | * Use alpha and gamma methods to make myoglobin GNPs | ||
==Procedure== | |||
<br> | |||
Alpha | |||
<br> | |||
<br> | |||
* Dilute 50 mg/mL myoglobin to 10^-6 M | |||
<br> | |||
Gamma | |||
<br> | |||
#A 1/10 solution of the reactant free nano particles was prepared from the stock solution | |||
#27mL of the AuNP solution was transferred to a beaker | |||
#1mL of 5mg/mL solution Myoglobin in H2O was added to the beaker | |||
#The mixture was stirred at room temperature for 30 minutes | |||
#The solution was then centrifuged for 30 minutes at 10,000rpm | |||
#The supernatant and pellet were separated | |||
#The pellet was resuspended in PBS solution with 0.1% BSA | |||
==Notes== | |||
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Revision as of 14:58, 22 April 2014
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March 18th
Procedure
Notes | |
