User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/03/04: Difference between revisions

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**stir for 30 minutes at room temperature
**stir for 30 minutes at room temperature
**spin for 30 minutes at 6500g
**spin for 30 minutes at 6500g
==data==
[[Image:Screen Shot 2014-03-18 at 1.26.17 PM.png]]


==Notes==
==Notes==

Revision as of 15:00, 22 April 2014

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March 4th

To DO

  1. determine concentration of gold particles
  2. find equal amount of reactant free gold particles

procedure

  • ran uv vis on our gold nanoparticles from alpha and beta to determine the concentration of our nanoparticles
    • they were determined to be around 10^12.
  • we are making the gamma method protein nanoparticles
    • doing 10^-8 M protein concentration
    • adding 9.9mls and 100ul of protein
    • stir for 30 minutes at room temperature
    • spin for 30 minutes at 6500g

data


Notes

The gold nanoparticle concentration that we were using in alpha and beta method were 10^12...The reactant free nps that we are using are 10^11, but we have diluted them tenfold to 10^10.

  • I think that we should have lesser activity in the gamma method as there is less gold nanoparticles per ml


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