User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/03/04

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 12: Line 12:
#find equal amount of reactant free gold particles
#find equal amount of reactant free gold particles
 +
==procedure==
 +
*ran uv vis on our gold nanoparticles from alpha and beta to determine the concentration of our nanoparticles
 +
**they were determined to be around 10^12.
 +
*we are making the gamma method protein nanoparticles
 +
**doing 10^-8 M protein concentration
 +
**adding 9.9mls and 100ul of protein
 +
**stir for 30 minutes at room temperature
 +
**spin for 30 minutes at 6500g
==Notes==
==Notes==

Revision as of 14:43, 4 March 2014

Project name Main project page
Previous entry      Next entry

March 4th

To DO

  1. determine concentration of gold particles
  2. find equal amount of reactant free gold particles

procedure

  • ran uv vis on our gold nanoparticles from alpha and beta to determine the concentration of our nanoparticles
    • they were determined to be around 10^12.
  • we are making the gamma method protein nanoparticles
    • doing 10^-8 M protein concentration
    • adding 9.9mls and 100ul of protein
    • stir for 30 minutes at room temperature
    • spin for 30 minutes at 6500g

Notes

The gold nanoparticle concentration that we were using in alpha and beta method were 10^12...The reactant free nps that we are using are 10^11, but we have diluted them tenfold to 10^10.

  • I think that we should have lesser activity in the gamma method as there is less gold nanoparticles per ml


Personal tools