User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/02/18

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Procedures)
(Notes)
Line 41: Line 41:
==Notes==
==Notes==
*HRP was 10^-8 M added to nanoparticles
*HRP was 10^-8 M added to nanoparticles
 +
<br>
 +
*when I made the new HRP particles, there was definitely some wierd impurities present...this is important to note if the HRP doesn't work
==Reflections==
==Reflections==

Revision as of 15:00, 18 February 2014

Project name Main project page
Previous entry      Next entry

February 18th

Goals

  • Make graph showing max peak over time
  • Making HRP nanoparticles using dopamine method
  • Run UV vis on HRP nanoparticles to see if there is any difference after having it run a week later
  • Run UV vis on HRP supernatant
    • We want to see there is free HRP in the solution and that all of the HRP did not get spun down



  • make hemoglobin nanoparticles using the alpha method

Procedures

Other things to do

  • take aliquot of 10^-8M HRP nanoparticles and put in freezer



  • Protein Gold Nanoparticles
    • I already have my 10^-6M Hemoglobin solution made
    • Take 1ml of the solution and add to 9ml of nanoparticles...take 100ul and add to 9.9mls of gold to make solutions


  • one solution was 10^-7M protein in nanoparticles. The other was 10^-8M.


Making HRP nanoparticles

    • First I made the buffer solution. The pH was 5. I made it using 0.0003 moles of acetic acid 1M and 0.0006 moles of potassium acetate
      • add 300ul of acid and 0.059g of base
    • Going to make 10^-6M HRP solution in the buffer
      • HRP molecular mass is 44kDa or 44000g/mol
        • added 0.0044g of HRP
      • we then filled up 100ml volumetric flask
    • Take 1ml of the solution and add to 9ml of nanoparticles...take 100ul and add to 9.9mls of gold to make solutions


  • one solution was 10^-7M protein in nanoparticles. The other was 10^-8

Notes

  • HRP was 10^-8 M added to nanoparticles


  • when I made the new HRP particles, there was definitely some wierd impurities present...this is important to note if the HRP doesn't work

Reflections


Personal tools