User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/02/04: Difference between revisions
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* | The goal of this experiment is to synthesize citrate protected gold nano particles of 40-45nm diameter. | ||
Dopamine solution will also be made to synthesize Polydopamine coated AuNPs. | |||
==Gold Nanoparticle Synthesis== | |||
==Procedure== | |||
#Prepare stock solutions | |||
##Dissolve HAuCl4 in water to have a solution of 10mM | |||
## Dissolve 250mg of the citrate sodium salt into 25mL of water | |||
#In a beaker mix 50mL of water and a volume of the gold stock solution corresponding to 1.214*10^-2 moles of Gold | |||
#Bring solution to a boil | |||
#Add a 0.5mL of the citrate sodium solution and reflux for 30 minutes | |||
##Note color change from dark blue to red | |||
==Polydopamine Coated AuNPs== | |||
#Dissolve dopamine in 100mL Tris Buffer to have a 10mM solution at pH=8.5 | |||
#Mix 17mL of AuNP solution with 17mL of the dopamine solution | |||
#Stir under air at room temperature for 1 hour | |||
#Purify by centrifuging and then redisperse in water. | |||
#Filtrate through syringe filters with cellulose acetate membranes with pore size of 1um | |||
#Make 2 solutions. | |||
## to be stored over night on lab bench | |||
## to be stored over night in the refrigerator | |||
Revision as of 10:32, 4 February 2014
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The goal of this experiment is to synthesize citrate protected gold nano particles of 40-45nm diameter. Dopamine solution will also be made to synthesize Polydopamine coated AuNPs. Gold Nanoparticle SynthesisProcedure
Polydopamine Coated AuNPs
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