User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/30

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Today we are making HRP and myoglobin and pepsin nanoparticles
Today we are making HRP and myoglobin and pepsin nanoparticles
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Table 1. Preparation of Myoglobin-AuNP solutions
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio Myoglobin'''
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| align="center" style="background:#f0f0f0;"|'''Amount of gold added(mL)'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Myoglobin(M)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Myoglobin(mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Water Myoglobin(mL)'''
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|-
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| 5||0.494264558||0.00005||2||2.505735442
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|-
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| 10||0.494264558||0.000025||1||3.505735442
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|-
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| 15||0.494264558||1.66667E-05||0.666666667||3.839068775
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|-
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| 20||0.494264558||0.0000125||0.5||4.005735442
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|-
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| 25||0.494264558||0.00001||0.4||4.105735442
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|-
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| 30||0.494264558||8.33333E-06||0.333333333||4.172402108
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|-
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| 35||0.494264558||7.14286E-06||0.285714286||4.220021156
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|-
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| 40||0.494264558||0.00000625||0.25||4.255735442
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|-
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| 45||0.494264558||5.55556E-06||0.222222222||4.28351322
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|-
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| 50||0.494264558||0.000005||0.2||4.305735442
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|}
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<br>
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Table 2. Preparation of all Lysozyme-AuNP solutions
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio Lysozyme'''
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| align="center" style="background:#f0f0f0;"|'''Amount gold added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Lysozyme(M)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Lysozyme (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Water Lysozyme (mL)'''
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|-
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| 30||0.494264558||8.33333E-06||1.611148649||2.894586793
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|-
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| 50||0.494264558||0.000005||0.966689189||3.539046253
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|-
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| 70||0.494264558||3.57143E-06||0.690492278||3.815243164
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|-
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| 90||0.494264558||2.77778E-06||0.53704955||3.968685892
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|-
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| 110||0.494264558||2.27273E-06||0.439404177||4.066331265
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|-
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| 130||0.494264558||1.92308E-06||0.371803534||4.133931907
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|-
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| 150||0.494264558||1.66667E-06||0.32222973||4.183505712
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|-
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| 170||0.494264558||1.47059E-06||0.28432035||4.221415092
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|-
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| 190||0.494264558||1.31579E-06||0.254391892||4.25134355
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|-
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| 210||0.494264558||1.19048E-06||0.230164093||4.275571349
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|}
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<br>
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Table 3. Stock Solution concentrations
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<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration of Myoglobin (M)'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Gold(M)'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Lysozyme (M)'''
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|-
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| 0.000125||0.00252901||2.58615E-05
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|}
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*All stock solutions were prepared with deionized H<sub>2</sub>O
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*The gold stock solution was prepared using Gold Chloride (II) trihydrate
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<br>
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Table 4. Preparation of Lysozyme Stock solution
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<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Molecular Weight(g/mol)'''
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| align="center" style="background:#f0f0f0;"|'''14307'''
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|-
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| mass lysozyme(g)||0.0037
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|-
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| volume solution(mL)||10
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|-
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| Concentration Lysozyme (M)||2.58615E-05
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|}
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<br>
 +
Table 5. Preparation of Myoglobin Stock solution
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Molecular weight (g/mol)'''
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| align="center" style="background:#f0f0f0;"|'''17600'''
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|-
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| mass myoglobin (g)||0.022
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|-
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| volume solution (mL)||0.01
 +
|-
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| Concentration (M)||0.000125
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|}
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<br>
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Revision as of 12:08, 5 November 2013

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Notes

Today we are making HRP and myoglobin and pepsin nanoparticles

Table 1. Preparation of Myoglobin-AuNP solutions

Ratio Myoglobin Amount of gold added(mL) Concentration Myoglobin(M) Volume Myoglobin(mL) Volume Water Myoglobin(mL)
50.4942645580.0000522.505735442
100.4942645580.00002513.505735442
150.4942645581.66667E-050.6666666673.839068775
200.4942645580.00001250.54.005735442
250.4942645580.000010.44.105735442
300.4942645588.33333E-060.3333333334.172402108
350.4942645587.14286E-060.2857142864.220021156
400.4942645580.000006250.254.255735442
450.4942645585.55556E-060.2222222224.28351322
500.4942645580.0000050.24.305735442


Table 2. Preparation of all Lysozyme-AuNP solutions

Ratio Lysozyme Amount gold added (mL) Concentration Lysozyme(M) Volume Lysozyme (mL) Volume Water Lysozyme (mL)
300.4942645588.33333E-061.6111486492.894586793
500.4942645580.0000050.9666891893.539046253
700.4942645583.57143E-060.6904922783.815243164
900.4942645582.77778E-060.537049553.968685892
1100.4942645582.27273E-060.4394041774.066331265
1300.4942645581.92308E-060.3718035344.133931907
1500.4942645581.66667E-060.322229734.183505712
1700.4942645581.47059E-060.284320354.221415092
1900.4942645581.31579E-060.2543918924.25134355
2100.4942645581.19048E-060.2301640934.275571349


Table 3. Stock Solution concentrations

Concentration of Myoglobin (M) Concentration Gold(M) Concentration Lysozyme (M)
0.0001250.002529012.58615E-05
  • All stock solutions were prepared with deionized H2O
  • The gold stock solution was prepared using Gold Chloride (II) trihydrate


Table 4. Preparation of Lysozyme Stock solution

Molecular Weight(g/mol) 14307
mass lysozyme(g)0.0037
volume solution(mL)10
Concentration Lysozyme (M)2.58615E-05


Table 5. Preparation of Myoglobin Stock solution

Molecular weight (g/mol) 17600
mass myoglobin (g)0.022
volume solution (mL)0.01
Concentration (M)0.000125



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