User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/22

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/10/22 Entry for User:Zachary_I._Mendel/Notebook/Zacks_Notebook)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==Objective==
-
* Insert content here...
+
# Synthesize Pepsin-AuNPs for reactivity tests tomorrow
 +
# Determine the Au:Protein ratio required for fiber formation for pepsin and HRP
 +
 
 +
==Description==
 +
# Synthesis of HRP-AuNPs and Pepsin-AuNPs
 +
## Into test tube that will contain a total of 5mL add
 +
## Au solution such that the final concentration is 0.25mM
 +
## Protein solution such that the final concentration reflects the following Au:Protein ratios
 +
### 30
 +
### 50
 +
### 70
 +
### 90
 +
### 110
 +
### 130
 +
### 150
 +
### 170
 +
### 190
 +
### 210
 +
## Water up to 5mL
 +
 
 +
==Data==
 +
Stock Solutions
 +
# HRP
 +
## 67.3mg in 100mL of water ---> 15.3 μM
 +
# Pepsin
 +
## 54.4mg in 100mL of water ---> 15.7 μM
 +
# Gold
 +
## Need 50mL of 2.5mM
 +
## 49.7mg of HAuCl<sub>4</sub>·3H<sub>2</sub>O in 50mL water --> 2.52 mM
 +
 
 +
==Notes==

Revision as of 12:44, 22 October 2013

Project name Main project page
Previous entry      Next entry

Objective

  1. Synthesize Pepsin-AuNPs for reactivity tests tomorrow
  2. Determine the Au:Protein ratio required for fiber formation for pepsin and HRP

Description

  1. Synthesis of HRP-AuNPs and Pepsin-AuNPs
    1. Into test tube that will contain a total of 5mL add
    2. Au solution such that the final concentration is 0.25mM
    3. Protein solution such that the final concentration reflects the following Au:Protein ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
    4. Water up to 5mL

Data

Stock Solutions

  1. HRP
    1. 67.3mg in 100mL of water ---> 15.3 μM
  2. Pepsin
    1. 54.4mg in 100mL of water ---> 15.7 μM
  3. Gold
    1. Need 50mL of 2.5mM
    2. 49.7mg of HAuCl4·3H2O in 50mL water --> 2.52 mM

Notes


Personal tools