User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/22: Difference between revisions
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== | ==Objective== | ||
# Synthesize Pepsin-AuNPs for reactivity tests tomorrow | |||
# Determine the Au:Protein ratio required for fiber formation for pepsin and HRP | |||
==Description== | |||
# Synthesis of HRP-AuNPs and Pepsin-AuNPs | |||
## Into test tube that will contain a total of 5mL add | |||
## Au solution such that the final concentration is 0.25mM | |||
## Protein solution such that the final concentration reflects the following Au:Protein ratios | |||
### 30 | |||
### 50 | |||
### 70 | |||
### 90 | |||
### 110 | |||
### 130 | |||
### 150 | |||
### 170 | |||
### 190 | |||
### 210 | |||
## Water up to 5mL | |||
==Data== | |||
Stock Solutions | |||
# HRP | |||
## 67.3mg in 100mL of water ---> 15.3 μM | |||
# Pepsin | |||
## 54.4mg in 100mL of water ---> 15.7 μM | |||
# Gold | |||
## Need 50mL of 2.5mM | |||
## 49.7mg of HAuCl<sub>4</sub>·3H<sub>2</sub>O in 50mL water --> 2.52 mM | |||
==Notes== | |||
Revision as of 09:44, 22 October 2013
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Objective
Description
DataStock Solutions
Notes |