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==Notes== | ==Notes== | ||
*The protocol for the synthesis of HRP-AuNP and Pepsin-AuNP can be found here [[User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/08/28]]. | |||
<br> | |||
Table 1. Preparation of Protein-AuNP solutions | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Ratio''' | |||
| align="center" style="background:#f0f0f0;"|'''Amount of gold added(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''concentration HRP(M)''' | |||
| align="center" style="background:#f0f0f0;"|'''concentration Pepsin(M)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume HRP(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Pepsin(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Water HRP (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Water Pepsin (mL)''' | |||
|- | |||
| 30||0.496031746||8.33333E-06||8.33333E-06||2.723311547||2.653927813||1.780656707||1.850040441 | |||
|- | |||
| 50||0.496031746||0.000005||0.000005||1.633986928||1.592356688||2.869981326||2.911611566 | |||
|- | |||
| 70||0.496031746||3.57143E-06||3.57143E-06||1.16713352||1.137397634||3.336834734||3.36657062 | |||
|- | |||
| 90||0.496031746||2.77778E-06||2.77778E-06||0.907770516||0.884642604||3.596197738||3.61932565 | |||
|- | |||
| 110||0.496031746||2.27273E-06||2.27273E-06||0.742721331||0.723798494||3.761246923||3.780169759 | |||
|- | |||
| 130||0.496031746||1.92308E-06||1.92308E-06||0.628456511||0.61244488||3.875511743||3.891523374 | |||
|- | |||
| 150||0.496031746||1.66667E-06||1.66667E-06||0.544662309||0.530785563||3.959305945||3.973182691 | |||
|- | |||
| 170||0.496031746||1.47059E-06||1.47059E-06||0.480584391||0.468340202||4.023383863||4.035628052 | |||
|- | |||
| 190||0.496031746||1.31579E-06||1.31579E-06||0.42999656||0.419041234||4.073971694||4.08492702 | |||
|- | |||
| 210||0.496031746||1.19048E-06||1.19048E-06||0.389044507||0.379132545||4.114923747||4.124835709 | |||
|} | |||
<br> | |||
*The varying ratios will be used to determine the ideal concentrations for the preparation of fibers of unraveled proteins. | |||
<br> | |||
*Absorbance of BSA-AuNPs was determined for future comparison of the activity of protein-AuNPs. | |||
<br> | |||
Figure 1. Absorbance spectra of a 1/10 dilution of BSA-AuNPs | |||
<br> | |||
[[Image:BSAAuNPsdilutionzem.png]] | |||
<br> | |||
*Absorbance of all starting materials used for synthesis of AuNPs today for future comparison | |||
<br> | |||
Figure 2. Absorbance spectra of a 1/10 dilution of 2.52mM gold in water | |||
<br> | |||
[[Image:Golddilutionzem.png]] | |||
<br> | |||
Figure 3. Absorbance spectra of 1/2 dilution of 15.3μM HRP | |||
<br> | |||
[[Image:HalfdilutedHRP10222013zem.png]] | |||
<br> | |||
Figure 4. Absorbance spectrum of 1/10 dilution of 15.6μM pepsin | |||
<br> | |||
[[Image:Pepsindilutionzem.png]] | |||
<br> | |||
*No absorbance peak was observed for pepsin. | |||
Revision as of 06:58, 28 October 2013
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Objective
Description
DataStock Solutions
Notes
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