User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/16: Difference between revisions
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== | ==Objective== | ||
We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol | |||
==Description== | |||
# Add experimental record here. Include what, how, and why... | |||
==Data== | |||
<u>Stock Solution</u> | |||
# Buffer | |||
## 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM | |||
# Luminol | |||
## Dissolve 12.9mg luminol in 300uL of DMSO | |||
## Add to 50mL buffer ---> 1.46mM | |||
# H2O2 | |||
## 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM | |||
## Check absorption at 250 [http://www.bio.net/mm/methods/1995-January/023533.html source] (ε(250) = 16.69 M<sup>-1</sup>cm<sup>-1</sup>). A = 0.7392. [H2O2] = 44.29mM | |||
==Notes== | |||
Revision as of 09:49, 16 October 2013
 Project name
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ObjectiveWe are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol Description
DataStock Solution
Notes | |
