User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/09: Difference between revisions
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==Notes== | ==Notes== | ||
Creating the EHNA stock | |||
- 5mg EHNA in 1mL DMSO ---> 15.9mM EHNA (1.9μL)...(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA | |||
- The reaction samples will contain roughly 1nM EHNA | |||
UV/vis | |||
Graph showing the changes in the concentrations of adenosine and inosine over time | |||
[[Image:Adenosine Concentrations vs. Time.png]] | |||
Adding the EHNA appeared to slow down the conversion of adenosine to inosine. | Adding the EHNA appeared to slow down the conversion of adenosine to inosine. | ||
Revision as of 11:45, 16 October 2013
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ObjectiveWe are running the same procedure as yesterday only with an inhibitor, EHNA. Description
DataEHNA stock
The reaction samples will contain roughly 1nM EHNA NotesCreating the EHNA stock - 5mg EHNA in 1mL DMSO ---> 15.9mM EHNA (1.9μL)...(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA - The reaction samples will contain roughly 1nM EHNA UV/vis Graph showing the changes in the concentrations of adenosine and inosine over time
Adding the EHNA appeared to slow down the conversion of adenosine to inosine. | |
