User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/08: Difference between revisions
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==Notes== | ==Notes== | ||
Table 1. Preparation of 0.0123M stock solution | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|Mass of adenosine (g) | |||
| align="center" style="background:#f0f0f0;"|0.0165 | |||
|- | |||
| Volume of buffer(mL)||5 | |||
|} | |||
<br> | |||
Table 2. Preparation of 40μM adenosine solution | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|Volume of stock(mL) | |||
| align="center" style="background:#f0f0f0;"|0.0098 | |||
|- | |||
| Volume of buffer(mL)||2.9902 | |||
|} | |||
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< | Figure 1. Corrected Absorbance spectra of the conversion of adenosine to inosine by ADA | ||
<br> | |||
[[Image:Adakinetics10082013zem.png]] | |||
Table 3. Molar Absorptivity of Adenosine at 260nm and Inosine at 250nm | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Wavelength''' | |||
| align="center" style="background:#f0f0f0;"|'''Adenosine''' | |||
| align="center" style="background:#f0f0f0;"|'''Inosine''' | |||
|- | |||
| 250||13333||11937.5 | |||
|- | |||
| 260||16866.7||7312.5 | |||
|} | |} | ||
<br> | |||
Figure 2. Monitoring concentration versus time of the conversion of adenosine to inosine using ADA as a catalyst. | |||
<br> | |||
[[Image:ConcvstimeadentoinoADAcatalystzem.png]] | |||
__NOTOC__ | __NOTOC__ |
Revision as of 11:47, 16 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||
ObjectiveTo observe and measure ADA turnover kinetics in the absence of an inhibitor. This work will be the basis of our comparison to ADA-AuNP turnover studies. Description
Data
NotesTable 1. Preparation of 0.0123M stock solution
|
Figure 1. Corrected Absorbance spectra of the conversion of adenosine to inosine by ADA
Table 3. Molar Absorptivity of Adenosine at 260nm and Inosine at 250nm
Wavelength | Adenosine | Inosine |
250 | 13333 | 11937.5 |
260 | 16866.7 | 7312.5 |
Figure 2. Monitoring concentration versus time of the conversion of adenosine to inosine using ADA as a catalyst.