User:Yeem/20.309/Mod 4 lab report: Difference between revisions
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[[Image:yeem-intestinemucus.jpg|thumb|right|'''Figure 5.''' Blue channel of mouse intestine image, corresponding to mucus.]] | [[Image:yeem-intestinemucus.jpg|thumb|right|'''Figure 5.''' Blue channel of mouse intestine image, corresponding to mucus.]] | ||
==Methods== | ==Methods== | ||
Jaewon's microscope used a 5-watt 532nm pulse laser to provide excitation. The image dimensions were 256x256 pixels, saved as unsigned 16-bit integers in separate channels. | Jaewon's microscope used a 5-watt 532nm pulse laser to provide excitation. The image dimensions were 256x256 pixels, saved as unsigned 16-bit integers in separate channels. The 3-D images were reconstructed using ImageJ's Volume Viewer plugin. | ||
==Discussion== | ==Discussion== | ||
===Beads=== | ===Beads=== |
Latest revision as of 09:42, 11 December 2007
Methods
Jaewon's microscope used a 5-watt 532nm pulse laser to provide excitation. The image dimensions were 256x256 pixels, saved as unsigned 16-bit integers in separate channels. The 3-D images were reconstructed using ImageJ's Volume Viewer plugin.
Discussion
Beads
The beads appeared elongated and slightly deformed, likely an artifact of the field of excitation.
Mouse intestine
A square area in the upper right (x-y plane) was photobleached by accident.