User:Xi Chen:SynBioLib: Difference between revisions
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==Parts== | ==Parts== | ||
===Replication origins=== | ===Replication origins=== | ||
*ColE1 | |||
*:To be added later (XC) | |||
*oriS | |||
*:1 / cell, controlled by repE gene and parABC partition. -- Learned from pETcoco (Novagen) | |||
*oriV | |||
*: 40 / cell, controlled by TrfA. -- learned from pETcoco (Novagen) | |||
===Constitutive promoters=== | ===Constitutive promoters=== | ||
===Inducible promoters=== | ===Inducible promoters=== | ||
Revision as of 21:39, 21 November 2011
General
Techniques
Gene/genome construction
SLIC/CPEC/Gibson
Error correction
Parts
Zinc-finger and TALE
Expected contributor: Adam
PUF
Expected contributor: Xi
Bacteria
Techniques
Cross-cell/cross-strain/cross-species DNA transfer
Expected contributor: Brian Renda
In vivo recombination
Expected contributor: PeterE, Erik, Jiri
Hosts
Phage as workhorse
Knockout strains and libraries
Parts
Replication origins
- ColE1
- To be added later (XC)
- oriS
- 1 / cell, controlled by repE gene and parABC partition. -- Learned from pETcoco (Novagen)
- oriV
- 40 / cell, controlled by TrfA. -- learned from pETcoco (Novagen)
Constitutive promoters
Inducible promoters
Note: Transfer functions
Terminators
RBS
Mobile genetic elements
Quorum sensing
Part organization/configuration
Impacts of spacers, orientation, promoter interferences, etc. on gene expression.
Circuits
Yeast
Techniques
Hosts
Parts
Circuits
Mammalian Cells
Techniques
Hosts
Parts
Inducible promoters
Question: Do bacterial ones work?
