User:Xi Chen:SynBioLib: Difference between revisions
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===Cross-cell/cross-strain/cross-species DNA transfer=== | ===Cross-cell/cross-strain/cross-species DNA transfer=== | ||
===In vivo recombination=== | ===In vivo recombination=== | ||
Expected contributor: PeterE, Erik, Jiri | |||
==Hosts== | ==Hosts== | ||
===Phage as workhorse=== | ===Phage as workhorse=== | ||
Revision as of 16:33, 18 November 2011
General
Techniques
Gene/genome construction
SLIC/CPEC/Gibson
Error correction
Parts
Zinc-finger and TALE
Expected contributor: Adam
PUF
Expected contributor: Xi
Bacteria
Techniques
Cross-cell/cross-strain/cross-species DNA transfer
In vivo recombination
Expected contributor: PeterE, Erik, Jiri
Hosts
Phage as workhorse
Knockout strains and libraries
Parts
Replication origins
Constitutive promoters
Inducible promoters
Note: Transfer functions
Terminators
RBS
Mobile genetic elements
Quorum sensing
Part organization/configuration
Impacts of spacers, orientation, promoter interferences, etc. on gene expression.
Circuits
Yeast
Techniques
Hosts
Parts
Circuits
Mammalian Cells
Techniques
Hosts
Parts
Inducible promoters
Question: Do bacterial ones work?
