User:Xi Chen:SynBioLib: Difference between revisions
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=General= | =General= | ||
==Techniques== | ==Techniques== | ||
===Gene/genome construction=== | |||
=Bacteria= | =Bacteria= | ||
==Techniques== | ==Techniques== | ||
| Line 17: | Line 18: | ||
==Hosts== | ==Hosts== | ||
==Parts== | ==Parts== | ||
===Light-inducible proteins=== | |||
==Circuits== | ==Circuits== | ||
| Line 39: | Line 41: | ||
PUF (Xi) | PUF (Xi) | ||
VP16 tricks | VP16 tricks | ||
Oligo-synthesis, Gibson assembly | Oligo-synthesis, Gibson assembly | ||
Utilizing in vivo DNA repair (error-prone polymerases) | Utilizing in vivo DNA repair (error-prone polymerases) | ||
In vivo and in vitro recombination | In vivo and in vitro recombination | ||
Revision as of 13:36, 18 November 2011
General
Techniques
Gene/genome construction
Bacteria
Techniques
Hosts
Phage as workhorse
Parts
Mobile genetic elements
Circuits
Yeast
Techniques
Hosts
Parts
Circuits
Mammalian Cells
Techniques
Hosts
Parts
Light-inducible proteins
Circuits
Impressive/legendary Circuits Bacteria
Mobile genetic elements (PeterE, Eric, Jiri) Cross-cell/cross-strain/cross-species DNA transfer Replication origins Terminators RBS Part organization/configuration (spacers, orientation, promoter interferences) Knockout strains, libraries Inducible gene expression Transfer functions Quorum sensing Mammalian Inducible promoters Do bacterial ones work? ZF/TALE (Adam) PUF (Xi) VP16 tricks
Oligo-synthesis, Gibson assembly
Utilizing in vivo DNA repair (error-prone polymerases)
In vivo and in vitro recombination
