User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/18: Difference between revisions
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==Summary== | ==Summary== | ||
* | * Loading 10% gel | ||
** Loading volume was corrected according to the quantification of previous blot to obtain equal loading | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''S0''' | |||
| align="center" style="background:#f0f0f0;"|'''S1''' | |||
| align="center" style="background:#f0f0f0;"|'''S2''' | |||
| align="center" style="background:#f0f0f0;"|'''S3''' | |||
| align="center" style="background:#f0f0f0;"|'''C0''' | |||
| align="center" style="background:#f0f0f0;"|'''C1''' | |||
| align="center" style="background:#f0f0f0;"|'''C2''' | |||
| align="center" style="background:#f0f0f0;"|'''C3''' | |||
|- | |||
| Loading volume (corrected)|||||||||||||||| | |||
|} | |||
* | |||
{| border="1" | |||
|+'''10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010''' | |||
! align="center" style="background:#f0f0f0;"|# | |||
! align="center" style="background:#f0f0f0;"|<u>1</u> | |||
! align="center" style="background:#f0f0f0;"|<u>2</u> | |||
! align="center" style="background:#f0f0f0;"|<u>3</u> | |||
! align="center" style="background:#f0f0f0;"|<u>4</u> | |||
! align="center" style="background:#f0f0f0;"|<u>5</u> | |||
! align="center" style="background:#f0f0f0;"|<u>6</u> | |||
! align="center" style="background:#f0f0f0;"|<u>7</u> | |||
! align="center" style="background:#f0f0f0;"|<u>8</u> | |||
! align="center" style="background:#f0f0f0;"|<u>9</u> | |||
! align="center" style="background:#f0f0f0;"|<u>10</u> | |||
! align="center" style="background:#f0f0f0;"|<u>11</u> | |||
! align="center" style="background:#f0f0f0;"|<u>12</u> | |||
! align="center" style="background:#f0f0f0;"|<u>13</u> | |||
! align="center" style="background:#f0f0f0;"|<u>14</u> | |||
! align="center" style="background:#f0f0f0;"|<u>15</u> | |||
|- | |||
!Gel<br>Lysates | |||
| align="center"|X | |||
| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (5 μL) | |||
| align="center"|HeLa -S<br>(40 μL) | |||
| align="center"|Jurkat <br>(40 μL) | |||
| align="center"|HEK293<br>(40 μL) | |||
| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (10 μL) | |||
| align="center"|S0<br>(10,1 μL) | |||
| align="center"|S1<br>(27,5 μL) | |||
| align="center"|S2<br>(10,8 μL) | |||
| align="center"|S3<br>(17,2 μL) | |||
| align="center"|C0<br>(40,0 μL) | |||
| align="center"|C1<br>(13,8 μL) | |||
| align="center"|C2<br>(38,3 μL) | |||
| align="center"|C3<br>(17,2 μL) | |||
| align="center"|X | |||
|- | |||
|} | |||
* Gel was cut in 4 pieces and treated with either GAPDH (1:2000, mouse), RII (1:500, rabbit), AKAP79 (1:500, mouse) or Gravin (1:500, rabbit) | |||
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==Materials & Methods== | ==Materials & Methods== | ||
Revision as of 05:35, 19 October 2010
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