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 Project name
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Summary
- Blocking membrane of 08October2010 → Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C
- Preparation of 2 10% Tris-glycine acrylamide gels
- Dilution of samples, corrected to lowest concentration
| Sample
|
μL Sample
|
μL dH2O
|
| HEK293 (12/08/2010) |
61,5 |
88,5
|
| HeLa -S (p17 (20.6.2010) |
26,0 |
124,0
|
| S0 (7.5.2010) |
236,9 |
0,0
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| S1 |
52,1 |
97,9
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| S2 |
150,0 |
0,0
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| S3 |
53,8 |
96,2
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| C0 (7.5.2010) |
2470,4 |
0,0
|
| C1 |
59,6 |
90,4
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| C2 |
36,1 |
113,9
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| C3 |
106,3 |
43,7
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10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
| #
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1
|
2
|
3
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4
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5
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6
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7
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8
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9
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10
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11
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12
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13
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14
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15
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Gel
Lysates
|
X
|
Fermantas high range Marker
(5 μL)
|
HEK293
(30 μL)
|
HeLa -S
(30 μL)
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Jurkat
(30 μL)
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Fermantas high range Marker
(10 μL)
|
S0
(30 μL)
|
S1
(30 μL)
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S2
(30 μL)
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S3
(30 μL)
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C0
(30 μL)
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C1
(30 μL)
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C2
(30 μL)
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C3
(30 μL)
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X
|
- Run at 200 V
- 10% gel was cut in 4 for
- GAPDH (1:2000, mouse (<40kDa))
- RIIα (1:1000, rabbit (40 - 75kDa))
- AKAP79 (1:1000, rabbit? (75 - 130kDa)) (Santa Cruz)
- AKAP250 (1:1000, Goat, (>130kDA)) (Santa Cruz)
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Project name
