User:Wilfred J. Poppinga/Notebook/Wilfreds Project/2009/08/13

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<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

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MBP-ArsR

MBP-ArsR fusion proteins
Colonies Name Plasmid Date of plating Antibiotics
0
 Self closer 50 μL pSB1AC3 12august2009 LB-Amp100
3
 Self closer 200 μL pSB1AC3 12august2009 LB-Amp100
1
 MBP-ArsR 50 μL pSB1AC3 12august2009 LB-Amp100
a twin colony (Two that have merged
 MBP-ArsR 200 μL pSB1AC3 12august2009 LB-Amp100

NOTE: The red numbers were zero colonies on this date, number stated here is the amount found on plate 14august2009 after been left on bench.

Metal promotors

Metal promotors
Colonies Name Plasmid Date of plating Antibiotics
0
 Cu+RBS 50 μL pSB1AC3 12august2009 LB-Amp100
0
 Cu+RBS 200 μL pSB1AC3 12august2009 LB-Amp100
0
 Cu-RBS 50 μL pSB1AC3 12august2009 LB-Amp100
TBD
 Cu-RBS 200 μL pSB1AC3 12august2009 LB-Amp100
TBD
 Cu+RBS 50 μL pSB3K3 12august2009 LB-Kan30
0
 Cu+RBS 200 μL pSB3K3 12august2009 LB-Kan30
0
 Cu-RBS 50 μL pSB3K3 12august2009 LB-Kan30
0
 Cu-RBS 200 μL pSB3K3 12august2009 LB-Kan30
0
 Zn+RBS 50 μL pSB1AC3 12august2009 LB-Amp100
0
 Zn+RBS 200 μL pSB1AC3 12august2009 LB-Amp100
0
 Zn-RBS 50 μL pSB1AC3 12august2009 LB-Amp100
0
 Zn-RBS 200 μL pSB1AC3 12august2009 LB-Amp100
18 & small cluster
 Zn+RBS 50 μL pSB3K3 12august2009 LB-Kan30
8
 Zn+RBS 200 μL pSB3K3 12august2009 LB-Kan30
?
 Zn-RBS 50 μL pSB3K3 12august2009 LB-Kan30
0
 Zn-RBS 200 μL pSB3K3 12august2009 LB-Kan30
0
 As+RBS 50 μL pSB1AC3 12august2009 LB-Amp100
?
 As+RBS 200 μL pSB1AC3 12august2009 LB-Amp100
0
 As-RBS 50 μL pSB1AC3 12august2009 LB-Amp100
0
 As-RBS 200 μL pSB1AC3 12august2009 LB-Amp100
0
 As+RBS 50 μL pSB3K3 12august2009 LB-Kan30
TBD
 As+RBS 200 μL pSB3K3 12august2009 LB-Kan30
1
 As-RBS 50 μL pSB3K3 12august2009 LB-Kan30
TBD
 As-RBS 200 μL pSB3K3 12august2009 LB-Kan30
Metal promotors with Paul's annealing
Colonies Name Plasmid Date of plating Antibiotics
0
 Cu-RBS 50 μL pSB1AC3 12august2009 LB-Amp100
0
 Cu-RBS 200 μL pSB1AC3 12august2009 LB-Amp100
2 very small
 Cu-RBS 50 μL pSB3K3 12august2009 LB-Kan30
1
 Cu-RBS 200 μL pSB3K3 12august2009 LB-Kan30
13
 Zn-RBS 50 μL pSB1AC3 12august2009 LB-Amp100
0
 Zn-RBS 200 μL pSB1AC3 12august2009 LB-Amp100
29 (various sizes)
 Zn-RBS 50 μL pSB3K3 12august2009 LB-Kan30
clustered, few single colonies
 Zn-RBS 200 μL pSB3K3 12august2009 LB-Kan30
0
 As-RBS 50 μL pSB1AC3 12august2009 LB-Amp100
0
 As-RBS 200 μL pSB1AC3 12august2009 LB-Amp100
4
 As-RBS 50 μL pSB3K3 12august2009 LB-Kan30
12, (incl. 2 big)
 As-RBS 200 μL pSB3K3 12august2009 LB-Kan30
0
 Selfcloser 50 μL pSB1AC3 12august2009 LB-Kan30
0
 Selfcloser 200 μL pSB1AC3 12august2009 LB-Kan30
1
 Selfcloser 50 μL pSB3K3 12august2009 LB-Kan30
7
 Selfcloser 200 μL pSB3K3 12august2009 LB-Kan30

NOTE: The red numbers were zero colonies on this date, number stated here is the amount found on plate 14august2009 after been left on bench.

Controls

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Controls
Colonies Name Plasmid Date of plating Antibiotics
∞
 † 50 μL pSB1AC3 12august2009 LB-Amp100
∞
 † 200 μL pSB1AC3 12august2009 LB-Amp100
0
 - 50 μL pSB1AC3 12august2009 LB-Amp100
0
 - 200 μL pSB1AC3 12august2009 LB-Amp100



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