User:Wesley J. Houston/Notebook/MAP/2013/03/26: Difference between revisions
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== | ==3/26== | ||
* | * Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit. | ||
* Determined yields of plasmids. | |||
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table --> | |||
|- | |||
| <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | |||
|- | |||
| 1. KAH201 (Plasmid) || 0.056 || 1.732 || 56.395 | |||
|- | |||
| 2. KAH201 (Plasmid) || 0.069 || 1.857 || 69.164 | |||
|} | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | |||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> || | |||
| rowspan="9" | [[Image:Electrophoresis6.jpg |150px|Results of the fifth electrophoresis attempt]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
|- | |||
| DNA (plasmid) || 25 μL | |||
|- | |||
| 10x buffer || 3.0 | |||
|- | |||
| enzyme 1 (Pst1) || 1.0 | |||
|- | |||
| enzyme 2 (Xba1 or Spe1)|| 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 0.0 μL | |||
|- | |||
| || 30 μL --> 37°C/ ~15 min. | |||
|} | |||
> Measure conc.'s of gel purified fragments | |||
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table --> | |||
|- | |||
| <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | |||
|- | |||
| 1. KAH201 (S/P) || 0.029 || 1.054 || 29.262 | |||
|- | |||
| 2. KAH201 (S/P) || 0.006 || 1.381 || 6.172 | |||
|- | |||
| 3. KAH182 (X/P) || -0.002 || 2 || -1.702 | |||
|- | |||
| 4. KAH182 (X/P) || 0.007 || 5.583 || 7.129 | |||
|} | |||
Revision as of 12:20, 31 March 2013
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3/26
> Measure conc.'s of gel purified fragments
|