User:Wesley J. Houston/Notebook/MAP/2013/03/25

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(3/25/13)
(3/25/13)
Line 11: Line 11:
|-valign="top"
|-valign="top"
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
-
| rowspan="9" | [[Image:Electrophoresis5.jpg‎ |150px|Results of the fourth electrophoresis attempt]]<br>15 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
+
| rowspan="9" | [[Image:Electrophoresis5.jpg‎ |150px|Results of the fourth electrophoresis attempt]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
|-
| DNA (plasmid) || 25 μL
| DNA (plasmid) || 25 μL

Revision as of 01:05, 26 March 2013

Project name Main project page
Previous entry      Next entry

3/25/13

Reagent Volume   Results of the fourth electrophoresis attempt
30 μL/lane, 1% agarose; Ladder
DNA (plasmid) 25 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 0.0 μL
  30 μL --> 37°C/ ~15 min.

> Measure conc.'s of gel purified fragments

Sample 260/280 ng/μL
1. KAH201 (S/P) 0.727 2.54
2. KAH182 (X/P) 0.789 13596



Personal tools