User:Wesley J. Houston/Notebook/MAP/2013/03/25: Difference between revisions
From OpenWetWare
(4 intermediate revisions by the same user not shown) | |||
Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==3/25/13== | ==3/25/13== | ||
* Prepped plasmids of KAH201 and determined concentration and purity. | |||
* Followed by digestion and gel electrophoresis, then purification. | |||
* However, concentrations were determined to be too low to complete a ligation. | |||
* More liquid cultures of KAH182 and KAH201 were prepped. | |||
** Colonies placed into LB broth overnight in shaking 37C incubator. | |||
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table --> | |||
|- | |||
| <u>Sample</u> || <u>260/280</u> || <u>ng/μL</u> | |||
|- | |||
| 1. KAH201 (Plasmid) || 1.795 || 54.054 | |||
|- | |||
| 2. KAH201 (Plasmid) || 1.708 || 47.244 | |||
|} | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | ||
|-valign="top" | |-valign="top" | ||
| <u>Reagent</u> || <u>Volume</u> || | | <u>Reagent</u> || <u>Volume</u> || | ||
| rowspan="9" | [[Image:Electrophoresis5.jpg |150px|Results of the fourth electrophoresis attempt]]<br> | | rowspan="9" | [[Image:Electrophoresis5.jpg |150px|Results of the fourth electrophoresis attempt]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA (plasmid) || 25 μL | | DNA (plasmid) || 25 μL | ||
Line 33: | Line 48: | ||
| 1. KAH201 (S/P) || 0.727 || 2.54 | | 1. KAH201 (S/P) || 0.727 || 2.54 | ||
|- | |- | ||
| 2. KAH182 (X/P) || 0.789 || | | 2. KAH182 (X/P) || 0.789 || 1.596 | ||
|} | |} | ||
* Due to the low concentrations, I made more liquid cultures of KAH182 and KAH201. | |||
** Followed the established protocol for making liquid cultures: pick one colony and place into LB broth overnight in shaking 37C incubator. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 12:12, 31 March 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||
3/25/13
> Measure conc.'s of gel purified fragments
|