User:Wesley J. Houston/Notebook/MAP/2013/03/25: Difference between revisions
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(Autocreate 2013/03/25 Entry for User:Wesley_J._Houston/Notebook/MAP) |
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{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | |||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> || | |||
| rowspan="9" | [[Image:Electrophoresis5.jpg |150px|Results of the fourth electrophoresis attempt]]<br>15 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
|- | |||
| DNA (plasmid) || 25 μL | |||
|- | |||
| 10x buffer || 3.0 | |||
|- | |||
| enzyme 1 (Pst1) || 1.0 | |||
|- | |||
| enzyme 2 (Xba1 or Spe1)|| 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 0.0 μL | |||
|- | |||
| || 30 μL --> 37°C/ ~15 min. | |||
|} | |||
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Revision as of 15:27, 25 March 2013
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