User:Wesley J. Houston/Notebook/MAP/2013/03/22

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(Autocreate 2013/03/22 Entry for User:Wesley_J._Houston/Notebook/MAP)
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==Entry title==
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==3/22/13==
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* Insert content here...
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{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
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|-valign="top"
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| <u>Reagent</u> || <u>Volume</u> || &nbsp;
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| rowspan="9" | [[Image:Electrophoresis4.jpg‎ |150px|Results of third electrophoresis]]<br>15 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
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| DNA (plasmid) || 20 μL
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| 10x buffer || 3.0
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| enzyme 1 (Pst1) || 1.0
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| enzyme 2 (Xba1 or Spe1)|| 1.0
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|-
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| dH<sub>2</sub>O || 5.0 μL
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| &nbsp; || 30 μL --> 37°C/ ~15 min.
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|}
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Revision as of 18:26, 25 March 2013

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3/22/13

Reagent Volume   Results of third electrophoresis
15 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 5.0 μL
  30 μL --> 37°C/ ~15 min.


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