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3/21/13
- Retrieved liquid cultures from the incubator.
- Pelleted the samples (3 mins @ 16.3k x g).
- No pellets of KAH201 were produced.
- KAH182 was successfully pelleted.
- Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
- Concentration and purity was also determined (43.201, 1.758 (1); 40.434, 1.751(2)).
- Note there were two samples of KAH182.
- Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
- Following the established protocol for bacterial transformation.
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