User:Wesley J. Houston/Notebook/MAP/2013/03/21

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(3/21/13)
Current revision (01:16, 26 March 2013) (view source)
(3/21/13)
 
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** KAH182 was successfully pelleted.
** KAH182 was successfully pelleted.
* Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
* Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
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** Concentration and purity was also determined (43.201, 1.758 (1); 40.434, 1.751(2)).
+
** Concentration and purity was also determined.
*** Note there were two samples of KAH182.
*** Note there were two samples of KAH182.
* Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
* Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
** Following the established protocol for bacterial transformation.
** Following the established protocol for bacterial transformation.
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{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
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|-
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| <u>Sample</u> || <u>260/280</u> || <u>ng/μL</u>
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|-
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| 1. KAH182 (Plasmid) || 1.758 || 43.201
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|-
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| 2. KAH182 (Plasmid) || 7.751 || 40.434
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|}
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3/21/13

  • Retrieved liquid cultures from the incubator.
  • Pelleted the samples (3 mins @ 16.3k x g).
    • No pellets of KAH201 were produced.
    • KAH182 was successfully pelleted.
  • Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
    • Concentration and purity was also determined.
      • Note there were two samples of KAH182.
  • Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
    • Following the established protocol for bacterial transformation.
Sample 260/280 ng/μL
1. KAH182 (Plasmid) 1.758 43.201
2. KAH182 (Plasmid) 7.751 40.434


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