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3/18/2013
- Retrying ligation of KAH201(S/P)/3403 + KAH182(X/P)/1675
> Ligations, following corrected version from 3/4/13, with 50 ng of vector instead of 25 ng.
| Ligation |
Plate results (lig : neg crtl) 03/18/13
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| 1. KAH201 (S/P)/3403, 50 ng + KAH182 (X/P)/1675, 50 ng |
TBD
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| 2. KAH201 (S/P)/3403, 50 ng |
TBD
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1 |
2 |
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| Insert DNA (KAH182) |
6.0 |
--- |
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| Vector DNA (KAH201) |
1.0 |
1.0 |
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| 2x lgn buf (Roche) |
8.0 |
5.0 |
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| T4 ligase (NEB) |
1.0 |
1.0 |
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| dH2O |
0 |
3.0 |
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16 μL |
10 μL |
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- Follow quick transformation protocol
- Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar
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