User:Wesley J. Houston/Notebook/MAP/2013/03/18: Difference between revisions
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==3/18/2013== | ==3/18/2013== | ||
* Retrying ligation of KAH201(S/P)/3403 + KAH182(X/P)/1675 | * Retrying ligation of KAH201(S/P)/3403 + KAH182(X/P)/1675 | ||
* Concentrations: KAH201 = 48.1 ng/uL, KAH1182 = 6.3 ng/uL | |||
** Concentrations were determined on 2/18/13 | |||
> Ligations, following corrected version from | > Ligations, following corrected version from 3/4/13, with 50 ng of vector instead of 25 ng. | ||
{| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | ||
| || 1 || 2 || | | || 1 || 2 || | ||
|- | |- | ||
| Insert DNA (KAH182)|| | | Insert DNA (KAH182)|| 8.0 || --- || | ||
|- | |- | ||
| Vector DNA (KAH201)|| 1.0 || 1.0 || | | Vector DNA (KAH201)|| 1.0 || 1.0 || | ||
|- | |- | ||
| 2x lgn buf (Roche) || | | 2x lgn buf (Roche) || 10.0 || 5.0 || | ||
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 || | | T4 ligase (NEB) || 1.0 || 1.0 || | ||
Line 31: | Line 25: | ||
| dH<sub>2</sub>O || 0 || 3.0 || | | dH<sub>2</sub>O || 0 || 3.0 || | ||
|- | |- | ||
| || | | || 20 μL || 10 μL || | ||
|} | |} | ||
Line 37: | Line 31: | ||
* Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar | * Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar | ||
{| class="wikitable" border="0" cellspacing="3" <!-- Ligations table --> | |||
| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/18/13</font> | |||
|- | |||
| 1. KAH201 (S/P)/<font color="blue">3403</font>, 50 ng + KAH182 (X/P)/1675, 50 ng || <font color="blue">0</font> | |||
|- | |||
| 2. KAH201 (S/P)/3403, 50 ng || 0 | |||
|} | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 22:13, 25 March 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||
3/18/2013
> Ligations, following corrected version from 3/4/13, with 50 ng of vector instead of 25 ng.
|