User:Wesley J. Houston/Notebook/MAP/2013/03/18
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* Retrying ligation of KAH201(S/P)/3403 + KAH182(X/P)/1675 | * Retrying ligation of KAH201(S/P)/3403 + KAH182(X/P)/1675 | ||
| - | > Ligations | + | > Ligations, following corrected version from [[3/4/13|http://openwetware.org/wiki/User:Wesley_J._Houston/Notebook/MAP/2013/02/08]], with 50 ng of vector, instead of 25 ng. |
{| class="wikitable" border="0" cellspacing="3" <!-- Ligations table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Ligations table --> | ||
| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/18/13</font> | | <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/18/13</font> | ||
| Line 17: | Line 17: | ||
| 2. KAH201 (S/P)/3403, 50 ng || TBD | | 2. KAH201 (S/P)/3403, 50 ng || TBD | ||
|} | |} | ||
| + | |||
| + | {| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | ||
| + | | || 1 || 2 || | ||
| + | |- | ||
| + | | Insert DNA (KAH182)|| 6.0 || --- || | ||
| + | |- | ||
| + | | Vector DNA (KAH201)|| 1.0 || 1.0 || | ||
| + | |- | ||
| + | | 2x lgn buf (Roche) || 5.0 || 5.0 || | ||
| + | |- | ||
| + | | T4 ligase (NEB) || 1.0 || 1.0 || | ||
| + | |- | ||
| + | | dH<sub>2</sub>O || 0 || 3.0 || | ||
| + | |- | ||
| + | | || 13 μL || 10 μL || | ||
| + | |} | ||
| + | |||
| + | * Follow quick transformation protocol | ||
| + | * Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar | ||
| + | |||
Revision as of 15:07, 18 March 2013
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3/18/2013
> Ligations, following corrected version from http://openwetware.org/wiki/User:Wesley_J._Houston/Notebook/MAP/2013/02/08, with 50 ng of vector, instead of 25 ng.
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