User:Wesley J. Houston/Notebook/MAP/2013/03/13: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Grew four liquid cultures of KAH182, following the same procedure (pick a colony and place into LB broth), in case this ligation attempt fails. | * Grew four liquid cultures of KAH182, following the same procedure (pick a colony and place into LB broth), in case this ligation attempt fails. | ||
> Ligations | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | ||
| || 1 || 2 || | | || 1 || 2 || | ||
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* Follow quick transformation protocol | * Follow quick transformation protocol | ||
* Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar | * Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar | ||
> Ligations (corrected version, 3/04/13) | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Ligations table --> | |||
| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/13/13</font> | |||
|- | |||
| 1. KAH201 (S/P)/<font color="blue">3403</font>, 25 ng + KAH182 (X/P)/1675, 26 ng || <font color="blue">0</font> | |||
|- | |||
| 2. KAH201 (S/P)/3403, 25 ng || 0 | |||
|} | |||
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Latest revision as of 22:32, 26 September 2017
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3/13/13
> Ligations
> Ligations (corrected version, 3/04/13)
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