User:Wesley J. Houston/Notebook/MAP/2013/03/13: Difference between revisions
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==3/13/13== | ==3/13/13== | ||
* | * Attempted ligation following the same protocol outlined on 3/4/13. | ||
* | * Grew four liquid cultures of KAH182, following the same procedure (pick a colony and place into LB broth), in case this ligation attempt fails. | ||
{| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | |||
| || 1 || 2 || | |||
|- | |||
| Insert DNA (KAH182) || 3.0 || --- || | |||
|- | |||
| Vector DNA (KAH201) || 0.5 || 0.5 || | |||
|- | |||
| 2x lgn buf (Roche) || 5.0 || 5.0 || | |||
|- | |||
| T4 ligase (NEB) || 1.0 || 1.0 || | |||
|- | |||
| dH<sub>2</sub>O || 0.5 || 3.5 || | |||
|- | |||
| || 10 μL || 10 μL || | |||
|} | |||
* Follow quick transformation protocol | |||
* Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar | |||
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Revision as of 13:07, 18 March 2013
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