User:Wesley J. Houston/Notebook/MAP/2013/03/13
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==3/13/13== | ==3/13/13== | ||
| - | * | + | * Attempted ligation following the same protocol outlined on 3/4/13. |
| - | * | + | * Grew four liquid cultures of KAH182, following the same procedure (pick a colony and place into LB broth), in case this ligation attempt fails. |
| + | {| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | ||
| + | | || 1 || 2 || | ||
| + | |- | ||
| + | | Insert DNA (KAH182) || 3.0 || --- || | ||
| + | |- | ||
| + | | Vector DNA (KAH201) || 0.5 || 0.5 || | ||
| + | |- | ||
| + | | 2x lgn buf (Roche) || 5.0 || 5.0 || | ||
| + | |- | ||
| + | | T4 ligase (NEB) || 1.0 || 1.0 || | ||
| + | |- | ||
| + | | dH<sub>2</sub>O || 0.5 || 3.5 || | ||
| + | |- | ||
| + | | || 10 μL || 10 μL || | ||
| + | |} | ||
| + | |||
| + | * Follow quick transformation protocol | ||
| + | * Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar | ||
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Revision as of 16:07, 18 March 2013
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