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3/11/13
- Grow liquid cultures of KAH182 to repeat ligation.
- Retry ligation with lower concentration plasmids.
> Ligations
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1 |
2 |
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| Insert DNA (KAH182) |
4.0 |
--- |
|
| Vector DNA (KAH201) |
0.65 |
0.65 |
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| 2x lgn buf (Roche) |
5.0 |
5.0 |
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| T4 ligase (NEB) |
1.0 |
1.0 |
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| dH2O |
0 |
3.5 |
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10 μL |
10 μL |
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- Follow quick transformation protocol
- Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar
| Ligation |
Plate results (lig : neg crtl) 03/11/13
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| 1. KAH201 (S/P)/3403, 25 ng + KAH182 (X/P)/1675, 26 ng |
0
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| 2. KAH201 (S/P)/3403, 25 ng |
0
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