User:Wesley J. Houston/Notebook/MAP/2013/03/08: Difference between revisions

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* All attempts proved unsuccessful.
* All attempts proved unsuccessful.


{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
> Ligations
|-valign="top"
{| class="wikitable" border="0" cellspacing="3" <!-- Ligations table -->
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/08/13</font>
| rowspan="9" |
|-
|-
| DNA (plasmid) || 20 μL
| 1. KAH201 (S/P)/<font color="blue">3403</font>, 25 ng + KAH182 (X/P)/1675, 26 ng || <font color="blue">0</font>
|-
|-
| 10x buffer || 3.0
| 2. KAH201 (S/P)/3403, 25 ng || 0
|-
| Enzyme 1 (Pst1) || 1.0
|-
| Enzyme 2 (Spe1 or Xba1) || 1.0
|-
| dH<sub>2</sub>O || 5.0 μL
|-
| &nbsp; || 30 μL --> 37°C/ ~15 min.
|}
|}


* No colonies were present in the ligation plate.


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Revision as of 22:08, 25 March 2013

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3/8/13

  • Ligation was repeated.
  • Attempted to grow more liquid cultures of ligated plasmids.
  • Attempted to confirm ligation by streaking the colonies on an agar plate.
  • All attempts proved unsuccessful.

> Ligations

Ligation Plate results (lig : neg crtl) 03/08/13
1. KAH201 (S/P)/3403, 25 ng + KAH182 (X/P)/1675, 26 ng 0
2. KAH201 (S/P)/3403, 25 ng 0
  • No colonies were present in the ligation plate.


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