User:Wesley J. Houston/Notebook/MAP/2013/03/08

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(3/8/13)
(3/8/13)
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* All attempts proved unsuccessful.
* All attempts proved unsuccessful.
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{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
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> Ligations
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|-valign="top"
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{| class="wikitable" border="0" cellspacing="3" <!-- Ligations table -->
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| <u>Reagent</u> || <u>Volume</u> || &nbsp;
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| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/08/13</font>
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| rowspan="9" |
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|-
|-
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| DNA (plasmid) || 20 μL
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| 1. KAH201 (S/P)/<font color="blue">3403</font>, 25 ng + KAH182 (X/P)/1675, 26 ng || <font color="blue">0</font>
|-
|-
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| 10x buffer || 3.0
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| 2. KAH201 (S/P)/3403, 25 ng || 0
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|-
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| Enzyme 1 (Pst1) || 1.0
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|-
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| Enzyme 2 (Spe1 or Xba1) || 1.0
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|-
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| dH<sub>2</sub>O || 5.0 μL
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|-
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| &nbsp; || 30 μL --> 37°C/ ~15 min.
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|}
|}
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* No colonies were present in the ligation plate.
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Revision as of 01:08, 26 March 2013

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3/8/13

  • Ligation was repeated.
  • Attempted to grow more liquid cultures of ligated plasmids.
  • Attempted to confirm ligation by streaking the colonies on an agar plate.
  • All attempts proved unsuccessful.

> Ligations

Ligation Plate results (lig : neg crtl) 03/08/13
1. KAH201 (S/P)/3403, 25 ng + KAH182 (X/P)/1675, 26 ng 0
2. KAH201 (S/P)/3403, 25 ng 0
  • No colonies were present in the ligation plate.


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