User:Wesley J. Houston/Notebook/MAP/2013/01/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Building a Reporter Gene </span> | ||
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== | ==Jan. 24, 2013== | ||
* | * Followed basic steps found [http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo here] for plasmid transfection. | ||
* Prepped samples: [http://partsregistry.org/Part:BBa_S04739 KAH182], [http://partsregistry.org/Part:BBa_S04745 KAH201], [http://partsregistry.org/Part:BBa_J176121 V0200] and a control. | |||
** Labeled as 1, 2, 3 and 4. | |||
* Samples were pipetted into sterile water. | |||
* These samples were the placed into a growth medium, Amp DH5α-T. | |||
** The control was simply used to ensure the samples did not become contaminated. | |||
* Next, they were placed into an incubator over night. | |||
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Revision as of 19:10, 28 January 2013
 Building a Reporter Gene
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Jan. 24, 2013
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