User:Wesley J. Houston/Notebook/MAP/2013/01/24

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(Autocreate 2013/01/24 Entry for User:Wesley_J._Houston/Notebook/MAP)
Current revision (21:10, 28 January 2013) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Building a Reporter Gene </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Jan. 24, 2013==
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* Insert content here...
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* Followed basic steps found [http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo here] for plasmid transfection.
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* Prepped samples: [http://partsregistry.org/Part:BBa_S04739 KAH182], [http://partsregistry.org/Part:BBa_S04745 KAH201], [http://partsregistry.org/Part:BBa_J176121 V0200] and a control.
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** Labeled as 1, 2, 3 and 4.
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* Samples were pipetted into sterile water.
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* These samples were the placed into a growth medium, Amp DH5α-T.
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** The control was simply used to ensure the samples did not become contaminated.
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* Next, they were placed into an incubator over night.
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Current revision

Building a Reporter Gene Main project page
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Jan. 24, 2013

  • Followed basic steps found here for plasmid transfection.
  • Prepped samples: KAH182, KAH201, V0200 and a control.
    • Labeled as 1, 2, 3 and 4.
  • Samples were pipetted into sterile water.
  • These samples were the placed into a growth medium, Amp DH5α-T.
    • The control was simply used to ensure the samples did not become contaminated.
  • Next, they were placed into an incubator over night.


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