User:Wesley J. Houston/Notebook/MAP/2013/01/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Building a Reporter Gene </span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Jan. 24, 2013== | ||
* | * Followed basic steps found [http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo here] for plasmid transfection. | ||
* Prepped samples: [http://partsregistry.org/Part:BBa_S04739 KAH182], [http://partsregistry.org/Part:BBa_S04745 KAH201], [http://partsregistry.org/Part:BBa_J176121 V0200] and a control. | |||
** Labeled as 1, 2, 3 and 4. | |||
* Samples were pipetted into sterile water. | |||
* These samples were the placed into a growth medium, Amp DH5α-T. | |||
** The control was simply used to ensure the samples did not become contaminated. | |||
* Next, they were placed into an incubator over night. | |||
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Latest revision as of 22:23, 26 September 2017
Building a Reporter Gene | Main project page Next entry |
Jan. 24, 2013
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