User:Vincent Rouilly/Hands On BioBrick Activity: Difference between revisions

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| colspan="1" style="background:#efefef;" | '''Repressed Promoter'''
| colspan="1" style="background:#efefef;" | '''Repressed Promoter'''
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| [[Image:HandsOnBioBrick_ConstitutivePromoter.png|400px|Constitutive Promoter Component]]
| [[Image:HandsOnBioBrick_ConstitutivePromoter.png|300px|Constitutive Promoter Component]]
| [[Image:HandsOnBioBrick_ActivatedPromoter.png|400px|Activated Promoter Component]]
| [[Image:HandsOnBioBrick_ActivatedPromoter.png|300px|Activated Promoter Component]]
| [[Image:HandsOnBioBrick_RepressedPromoter.png|400px|Repressed Promoter Component]]
| [[Image:HandsOnBioBrick_RepressedPromoter.png|300px|Repressed Promoter Component]]
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|-   
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| colspan="1" style="background:#efefef;" | '''Stop Codon'''
| colspan="1" style="background:#efefef;" | '''Stop Codon'''
|-
|-
| [[Image:HandsOnBioBrick_RBS.png|400px|Constitutive Promoter Component]]
| [[Image:HandsOnBioBrick_RBS.png|300px|Constitutive Promoter Component]]
| [[Image:HandsOnBioBrick_ProteinCodingRegion.png|400px|Activated Promoter Component]]
| [[Image:HandsOnBioBrick_ProteinCodingRegion.png|300px|Activated Promoter Component]]
| [[Image:HandsOnBioBrick_StopCodon.png|400px|Repressed Promoter Component]]
| [[Image:HandsOnBioBrick_StopCodon.png|300px|Repressed Promoter Component]]
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|-   
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* a 10-LEDs graph bar is used to indicate the level of expression.
* a 10-LEDs graph bar is used to indicate the level of expression.
* a LED is used separately to potentially drive another promoter through a fiber optic.  
* a LED is used separately to potentially drive another promoter through a fiber optic.  
* the Resistor-Capacitor is used to add a delay.
|
|
* The stop codon enables the circuit to connect to earth through the table
* The stop codon enables the circuit to connect to earth through the table
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Revision as of 11:25, 6 June 2007

Vincent 13:42, 6 June 2007 (EDT): feel free to comment on the content of the activity described and its design.

Context

  • Following last year | Imperial College participation to iGEM, my group is putting together an exhibit for the Royal Society Summer Science Exhibition 2007.
  • We feel it is a great opportunity to engage with the Public on the concepts behind Synthetic Biology.
  • The exhibit attracts a diverse audience from school kids to science enthusiasts.
  • I have started to design a hands-on activity, and describe its early design below.

Intentions behind the activity

  • To set the background in terms of Molecular and Celluar Biology:
    • DNA, Central Dogma, Cells and Bacteria
    • Genetic Engineering techniques
  • To touch on Synthetic Biology concepts:
    • BioBricks
    • Modularity
    • Re-usability
  • To give an opportunity to build simple genetic circuits based on opto-electronic components:
    • Protein Generator
    • Inverter, Toggle switch & Repressilator
    • Link circuits with inputs such as: temperature sensor, Infra-red sensor, Touch sensor, Humidity sensor.
    • Link circuits with outputs such as: flagella motor, secretion fan.

Overview

Overview General setting
Hands on BioBrick table Hands on BioBrick IO modules
  • the activity stands on an oval table, decorated as an E.Coli bacteria.
  • on the right side of the table, DNA, gene expression machinery are illustrated.
  • the hands on activity is placed on the left side:
    • a set of available biobricks implemented as Lego bricks filled with some opto-electronic components.
    • a conductive surface
    • a set of fiber optics to link the different genetic circuits.
  • the activity offers a set of possible inputs for the genetic circuits to be built:
    • temperature sensor (to illustrate heat shock response).
    • infra-red sensor - with remote - (to illustrate UV response)
    • touch sensor
    • humidity sensor
  • 2 outputs are also available:
    • motor driving some flagella
    • fan to illustrate secretion


Components and Schematics

Constitutive Promoter Activated Promoter Repressed Promoter
Constitutive Promoter Component Activated Promoter Component Repressed Promoter Component
  • Strength of the promoter is set through the potentiometer
  • A battery is embedded into the lego brick
  • The Lego brick is sealed with a conductive plate to make contact with the table.
  • Activity of the promoter is driven by the amount of light received on the photo-sensor.
  • A battery is embedded into the lego brick
  • The Lego brick is sealed with a conductive plate to make contact with the table.
  • Activity of the promoter is driven by the amount of light received on the photo-sensor.
  • A battery is embedded into the lego brick
  • The Lego brick is sealed with a conductive plate to make contact with the table.
RBS Protein Coding Region Stop Codon
Constitutive Promoter Component Activated Promoter Component Repressed Promoter Component
  • Strength of the RBS is set through the potentiometer
  • a 10-LEDs graph bar is used to indicate the level of expression.
  • a LED is used separately to potentially drive another promoter through a fiber optic.
  • the Resistor-Capacitor is used to add a delay.
  • The stop codon enables the circuit to connect to earth through the table
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