User:Victor Tapia

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Contact Info

I work in the Inst. f. Med. Immunol. at the Charité Medical School of Berlin in the research group of Dr. R. Volkmer [1,2].


Education

  • 2009?, PhD, Institute for Medical Immunology, Charité Medical School, Berlin
  • 2004, Biology Diploma, Humboldt University, Berlin

Research interests

Publications

  • 4 - Tapia VE & Volkmer R (2009) "EXPLORING AND PROFILING PROTEIN FUNCTION WITH PEPTIDE ARRAYS" in: Methods in Molecular Biology, Vol 570 (Eds. M Cretich & M Chiari). Humana Press. - ISBN: 978-1-60327-393-0.
    This work reviews the peptide array technology focusing on technological advancements, oriented immobilization of peptide probes, and some interesting applications. Going along the philosophy of the monography series, we give some general recomendations on methods and instruments.
  • 3 - Tapia VE, Ay B, Triebus J, Wolter E, Boisguerin P, Volkmer R. EVALUATING THE COUPLING EFFICIENCY OF PHOSPHORYLATED AMINO ACIDS FOR SPOT SYNTHESIS. J Pept Sci. 2008 Dez ;14(12):1309-1314. - PMID: 18816512.
    This work was carried out to adapt "building block" approaches for the generation of phosphopeptides through resine-supported solid-phase peptide synthesis to the spot-technology, which relies on cellulose as support. It compares different coupling strategies for the incorporation of protected phoshoaminoacids into determined positions of synthetic peptides. We find that the use of EEDQ (caution, neurotoxic!) as coupling activator is the most efficient strategy in our comparison.
  • 2 - Tapia VE, Bongartz J, Schutkowski M, Bruni N, Weiser A, Ay B, Volkmer R, Or-Guil M. AFFINITY PROFILING USING THE PEPTIDE MICROARRAY TECHNOLOGY: A CASE STUDY. Anal Biochem. 2007 Apr 1;363(1):108-118. - PMID: 17288979.
    This work analyses the predictive potential of microarray-based binding assays. Fluorescent SI-measurements were used to fit a mass-action derived model estimating the observed affinity (equil. diss. const.).
  • 1 - Weiser AA, Or-Guil M, Tapia VE, Leichsenring A, Schuchhardt J, Frommel C, Volkmer-Engert R. SPOT SYNTHESIS: RELIABILITY OF ARRAY-BASED MEASUREMENT OF PEPTIDE BINDING AFFINITY. Anal Biochem. 2005 Juli 15;342(2):300-311. - PMID: 15950918.
    This work analyses the quantitative potential of measurements derived from peptide macroarray-based immunobinding assays. Chemoluminiscent signal intensity data was confronted with independent "golden stardard" measurements (surface plasmon resonance-based binding assays on a BIAcoreX maschine).


Presentations

  • 5 - V.E. Tapia & R. Volkmer; Steady State Analysis of Peptide Array-based Binding Assays: A Mathematical Model for Disease Diagnostic and Affinity Profiling of Bimolecular Interactions. 9th German Peptide Symposium, 2009, Göttingen.
  • 4 - V.E. Tapia, C. Landgraf, A. Pastore, M. Sudol, R. Volkmer; Analysis of a missense mutation in the WW domain of PQBP1. FEBS Workshop 2007, Protein Modules and Networks in Health and Disease, Seefeld, Austria.
  • 3 - V.E. Tapia et al; titel; 2008 Brownschweig, Germany
  • 2 - V.E. Tapia et al; titel; 2007 Frankfurt, Germany
  • 1 - V.E. Tapia & M. Or-Guil; Reading Natural Autoimmune Responses with Random Peptide Arrays; Workshop "Strukturbildung in Chemie und Biophysik", Salzwedel, Germany, October 2004.


Useful links

Who's visiting

start on 16.03.2009

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Science in the city

This is a short series of high content essays about the every day life
 of scientists in berlin. If you really have the time, or your boss is
not around at the moment, then read ahead and enjoy.
DAY 1


        was just returning from an international symposium and, naturally, the first thing i did once in berlin was to go to the lab and get some very interesting things done. It was not necesary to do it right away, but my sense of responsability was taking over me. At the moment, i did not know that this desition was about to change my whole carreer.

        But i only made it to the stairs because a bunch of squared-headed guys intercepted me on the way. They were all very disappointed about the attitude of scientists, saying: "these people are always thinking about cheap prices and bonus".

        As it happened: they all belonged to the provider-rebel-army and wanted me to go with them on a special mision. I thought they were quite funny, so i complied. Since then, they have been treating me very nicely. And i have total freedom of speech, as you can see. They took this nice fotograph in the case anybody would be missing me. To call their attention, they said.

DE >> war gerade wieder von einem internationalen Symposium zurück und, natürlich, das erste, was ich einmal in Berlin gemacht habe, war ins Labor zu gehen um einige sehr interessante Dinge zu erledigen. Es war nicht notwendig, es so unmittelbar zu tun, aber mein Gefühl der Verantwortung war stärker als ich. In diesem Moment wusste ich nicht, dass diese Entscheidung dabei war, meine ganze Karriere zu lenken.
Aber ich habe es nur bis zur Treppe geschafft, da eine Reihe von Viereck-köpfigen Jungs mich auf den Weg fing. Sie waren alle sehr enttäuscht über die Haltung der Wissenschaftler. Sie sagten: "Diese Menschen denken immer nur über günstigen Preisen und Bonus".
So wie es geschah: sie alle gehörten zu den Provider-Rebellen-Armee und wollten, dass ich mit ihnen auf eine besondere Mission gehe. Ich dachte, sie waren ganz lustig und war also einverstanden. Seit dann behandeln sie mich sehr gut. Und ich habe die totale Rede-Freiheit, wie man sehen kann. Sie haben dieses schöne Fotograph aufgenommen, falls irgendjemand mich vermissen sollte. Um ihre Aufmerksamkeit zu locken, sagten sie. <<


DAY 2


        The results of this week are astonishing. Firstly, I have found that not every squared-headed is really what I supposed to. I can report of 20 little guys, which like to stick covalently together, and a bottle of acetonitrile. I am sure that they are not squared-like at all. My new friends also complained about the way they have been treated: forced to remain on a fixed table for matters of order, to be bathed in acids and toxic solvents. Acetonitrile said that this was true. Indeed, she herself was forced on repeated times to deprive them from water. Every time acetonitrile came, they had to rapidly elute from there. What followed after this was a plethora of tortures.

        It is difficult to tell you about all I’ve seen, I won’t. It would not be fair to upset you in such a way with the sorrow. Instead I can tell you that we have organized ourselves and we are not going to take it anymore. We have broken out on Friday with the fabulous help of the nice Katja. She happily agreed to help us but resisted to come along. Katja didn’t know how her cell cultures would survive the whole struggle which was waiting in the city for all which dare to runaway. From the beginning we were quite aware about the risks, but were determined to be free. She gave us farewell and a smile as she threw us into the sky of Berlin.

DE >> Die Ergebnisse dieser Woche sind erstaunlich. Erstens, ich habe festgestellt, dass nicht jeder Viereck-Köpfige wirklich das ist, was ich bis jetzt angenommen habe. Ich kann von 20 kleinen Jungs berichten, die gerne kovalent zusammenhängen, und von eine Acetonitril Flasche. Ich bin‘s mir sicher, dass sie überhaupt nicht Viereck-artig sind. Meine neuen Freunde beschweren sich auch über die Art, wie sie behandelt worden sind: gezwungen starr auf irgendwelche Tabellen zu posieren, gebadet in Säuren und giftige Lösungsmittel, usw. Acetonitril, sagte, dies sei wahr. Tatsächlich, sie selbst war wiederholt gezwungen, ihnen das Wasser zu entziehen. Jedes Mal, dass Acetonitril kam, mussten sie schnell von dannen eluieren. Was dann folgte, war eine Vielzahl von Folterungen.

Es ist schwer darüber, was ich gesehen habe, zu erzählen. Und ich werde es nicht tun. Es wäre nicht fair, Euch in einer solchen Art und Weise mit der Trauer zu verstimmen. Stattdessen kann ich Euch sagen, dass wir uns selbst organisiert haben, und wir sind nicht mehr zu nehmen. Wir sind am Freitag ausgebrochen, mit der fantastischen Unterstützung der schönen Katja. Sie war bereit uns zu helfen, aber wiederstand unsere bitten mit uns zu kommen. Katja wusste nicht, wie ihre Zellkulturen den gesamten Kampf überleben würden, die in der Stadt auf alle wartete, die es wagen, zu fliehen. Von Anfang an waren wir sehr wohl über die Risiken bewusst, aber auch stärker bestimmt, frei zu sein. Sie gab uns Abschied und ein Lächeln, als sie uns in den Himmel von Berlin warf. <<


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